184082

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4608046..4608543 Replicon chromosome
Accession NZ_CP066144
Organism Escherichia coli strain ZLWT

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ICI43_RS22090 Protein ID WP_001173343.1
Coordinates 4608046..4608219 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag ICI43_RS22095 Protein ID WP_001312331.1
Coordinates 4608247..4608543 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICI43_RS22080 4604716..4606173 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
ICI43_RS22085 4606410..4607927 + 1518 WP_000003806.1 lysine--tRNA ligase -
ICI43_RS22090 4608046..4608219 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ICI43_RS22095 4608247..4608543 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ICI43_RS22100 4608772..4609044 - 273 WP_000405647.1 N-acetyltransferase -
ICI43_RS22105 4609056..4609286 - 231 WP_000371715.1 (4Fe-4S)-binding protein -
ICI43_RS22110 4609467..4611098 + 1632 WP_001216466.1 sensor histidine kinase -
ICI43_RS22115 4611095..4611814 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T184082 WP_001173343.1 NZ_CP066144:c4608219-4608046 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T184082 NZ_CP087329:c23049-22693 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT184082 WP_001312331.1 NZ_CP066144:c4608543-4608247 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT184082 NZ_CP087329:c22700-22398 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAACTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References