Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4232217..4232767 Replicon chromosome
Accession NZ_CP066144
Organism Escherichia coli strain ZLWT

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag ICI43_RS20260 Protein ID WP_001160966.1
Coordinates 4232217..4232531 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ICI43_RS20265 Protein ID WP_000125566.1
Coordinates 4232534..4232767 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICI43_RS20235 4227892..4229178 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
ICI43_RS20240 4229178..4230167 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ICI43_RS20245 4230164..4230985 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ICI43_RS20250 4230988..4231365 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ICI43_RS20255 4231372..4232220 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ICI43_RS20260 4232217..4232531 - 315 WP_001160966.1 CcdB family protein Toxin
ICI43_RS20265 4232534..4232767 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ICI43_RS20270 4232853..4233332 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
ICI43_RS20275 4233524..4235386 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
ICI43_RS20280 4235379..4235909 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ICI43_RS20285 4236017..4237348 - 1332 WP_001183207.1 MFS transporter -
ICI43_RS20290 4237407..4237694 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T184076 WP_001160966.1 NZ_CP066144:c4232531-4232217 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T184076 NZ_CP087326:83623-83979 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT184076 WP_000125566.1 NZ_CP066144:c4232767-4232534 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT184076 NZ_CP087326:83972-84274 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAACTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References