Detailed information of TA system TA184070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2557251..2558839
• Replicon: chromosome
• Accession: NZ_CP066144
• Organism: Escherichia coli strain ZLWT

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1L1KDK2
• Locus tag: ICI43_RS12085
• Protein ID: WP_001125431.1
• Coordinates: 2557517..2558839 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: ICI43_RS12080
• Protein ID: WP_001304368.1
• Coordinates: 2557251..2557517 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI43_RS12065	2553619..2555070	+	1452	WP_000854660.1	tagaturonate reductase	-
ICI43_RS12070	2555267..2556181	+	915	WP_001443133.1	bestrophin family protein	-
ICI43_RS12075	2556185..2556943	-	759	WP_001286540.1	trans-aconitate 2-methyltransferase	-
ICI43_RS12080	2557251..2557517	+	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ICI43_RS12085	2557517..2558839	+	1323	WP_001125431.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
ICI43_RS12090	2559098..2559511	-	414	WP_000753076.1	acetylxylan esterase	-
ICI43_RS12095	2559559..2560140	-	582	Protein_2369	LysR family transcriptional regulator	-
ICI43_RS12100	2560289..2561071	+	783	WP_001221834.1	SDR family oxidoreductase	-
ICI43_RS12105	2561847..2562410	+	564	WP_001327671.1	type 1 fimbrial major subunit FimA	-
ICI43_RS12110	2562772..2563482	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49310.02 Da
• Isoelectric Point: 8.6972

>T184070 WP_001125431.1 NZ_CP066144:2557517-2558839 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T184070 NZ_CP087323:10646-11002 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCTGATCAGCAATACGAACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT184070 WP_001304368.1 NZ_CP066144:2557251-2557517 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT184070 NZ_CP087323:10995-11297 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCTGATGAATT
ACTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCGTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACCATGAAAAAGTATGTTGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A1L1KDK2

Antitoxin

• AlphaFold DB: V0VAH2