Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2557251..2558839 Replicon chromosome
Accession NZ_CP066144
Organism Escherichia coli strain ZLWT

Toxin (Protein)


Gene name hipA Uniprot ID A0A1L1KDK2
Locus tag ICI43_RS12085 Protein ID WP_001125431.1
Coordinates 2557517..2558839 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag ICI43_RS12080 Protein ID WP_001304368.1
Coordinates 2557251..2557517 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICI43_RS12065 2553619..2555070 + 1452 WP_000854660.1 tagaturonate reductase -
ICI43_RS12070 2555267..2556181 + 915 WP_001443133.1 bestrophin family protein -
ICI43_RS12075 2556185..2556943 - 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
ICI43_RS12080 2557251..2557517 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
ICI43_RS12085 2557517..2558839 + 1323 WP_001125431.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
ICI43_RS12090 2559098..2559511 - 414 WP_000753076.1 acetylxylan esterase -
ICI43_RS12095 2559559..2560140 - 582 Protein_2369 LysR family transcriptional regulator -
ICI43_RS12100 2560289..2561071 + 783 WP_001221834.1 SDR family oxidoreductase -
ICI43_RS12105 2561847..2562410 + 564 WP_001327671.1 type 1 fimbrial major subunit FimA -
ICI43_RS12110 2562772..2563482 + 711 WP_001195181.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49310.02 Da        Isoelectric Point: 8.6972

>T184070 WP_001125431.1 NZ_CP066144:2557517-2558839 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T184070 NZ_CP087323:10646-11002 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCTGATCAGCAATACGAACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT184070 WP_001304368.1 NZ_CP066144:2557251-2557517 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT184070 NZ_CP087323:10995-11297 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCTGATGAATT
ACTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCGTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACCATGAAAAAGTATGTTGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L1KDK2

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References