Detailed information of TA system TA184054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 5128323..5129136
• Replicon: chromosome
• Accession: NZ_CP066143
• Organism: Klebsiella pneumoniae U-0608239

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: I6N99_RS25225
• Protein ID: WP_101516303.1
• Coordinates: 5128600..5129136 (+)
• Length: 179 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: I6N99_RS25220
• Protein ID: WP_023343051.1
• Coordinates: 5128323..5128589 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6N99_RS25200 (5124194)	5124194..5124538	-	345	WP_004192220.1	cation efflux system protein CusF	-
I6N99_RS25205 (5124556)	5124556..5125941	-	1386	WP_040182471.1	efflux transporter outer membrane subunit	-
I6N99_RS25210 (5126114)	5126114..5126797	+	684	WP_002887273.1	copper response regulator transcription factor CusR	-
I6N99_RS25215 (5126787)	5126787..5128220	+	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
I6N99_RS25220 (5128323)	5128323..5128589	+	267	WP_023343051.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
I6N99_RS25225 (5128600)	5128600..5129136	+	537	WP_101516303.1	type II toxin-antitoxin system toxin KacT	Toxin
I6N99_RS25230 (5129184)	5129184..5130305	-	1122	WP_012737335.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 179 a.a.
• Molecular weight: 19951.87 Da
• Isoelectric Point: 5.7068

>T184054 WP_101516303.1 NZ_CP066143:5128600-5129136 [Klebsiella pneumoniae U-0608239]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSN
LLFYPTKSIEQLFTDDES

Nucleotide

• Length: 537 bp

>T184054 NZ_CP087311:c54261-53902 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT184054 WP_023343051.1 NZ_CP066143:5128323-5128589 [Klebsiella pneumoniae U-0608239]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT184054 NZ_CP087311:c53902-53603 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA