Detailed information of TA system TA184051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4565405..4566030
• Replicon: chromosome
• Accession: NZ_CP066143
• Organism: Klebsiella pneumoniae U-0608239

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6N99_RS22600
• Protein ID: WP_040182550.1
• Coordinates: 4565647..4566030 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: I6N99_RS22595
• Protein ID: WP_004150355.1
• Coordinates: 4565405..4565647 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6N99_RS22570 (4561396)	4561396..4561995	+	600	WP_004151865.1	glucose-1-phosphatase	-
I6N99_RS22575 (4561989)	4561989..4562849	+	861	WP_004146232.1	virulence factor BrkB family protein	-
I6N99_RS22580 (4562846)	4562846..4563283	+	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
I6N99_RS22585 (4563328)	4563328..4564269	+	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
I6N99_RS22590 (4564283)	4564283..4565200	-	918	WP_023302328.1	alpha/beta hydrolase	-
I6N99_RS22595 (4565405)	4565405..4565647	+	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
I6N99_RS22600 (4565647)	4565647..4566030	+	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6N99_RS22605 (4566204)	4566204..4567133	-	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
I6N99_RS22610 (4567130)	4567130..4567765	-	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
I6N99_RS22615 (4567762)	4567762..4568664	-	903	WP_002882822.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T184051 WP_040182550.1 NZ_CP066143:4565647-4566030 [Klebsiella pneumoniae U-0608239]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T184051 NZ_CP087304:449934-450323 [Acinetobacter baumannii]
TTGCTCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATAAGCTTTATTTTCTTTT
TAAAGCGTCCGCAACTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT184051 WP_004150355.1 NZ_CP066143:4565405-4565647 [Klebsiella pneumoniae U-0608239]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT184051 NZ_CP087304:449670-449927 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9