Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 1885744..1887332 Replicon chromosome
Accession NZ_CP066143
Organism Klebsiella pneumoniae U-0608239

Toxin (Protein)


Gene name hipA Uniprot ID A0A486S9S0
Locus tag I6N99_RS09260 Protein ID WP_023301940.1
Coordinates 1886010..1887332 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag I6N99_RS09255 Protein ID WP_002904790.1
Coordinates 1885744..1886010 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6N99_RS09230 (1880894) 1880894..1881817 + 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -
I6N99_RS09235 (1881798) 1881798..1882952 - 1155 WP_040182036.1 FAD-dependent urate hydroxylase HpxO -
I6N99_RS09240 (1883298) 1883298..1884677 + 1380 WP_004176204.1 nucleobase:cation symporter-2 family protein -
I6N99_RS09245 (1884655) 1884655..1885155 + 501 WP_040182039.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
I6N99_RS09250 (1885152) 1885152..1885478 + 327 WP_004143476.1 hydroxyisourate hydrolase -
I6N99_RS09255 (1885744) 1885744..1886010 + 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
I6N99_RS09260 (1886010) 1886010..1887332 + 1323 WP_023301940.1 type II toxin-antitoxin system HipA family toxin Toxin
I6N99_RS09265 (1887349) 1887349..1888641 - 1293 WP_016946381.1 glycoside hydrolase family 10 protein -
I6N99_RS09270 (1888783) 1888783..1889787 - 1005 WP_009308876.1 fimbrial protein -
I6N99_RS09275 (1889775) 1889775..1892276 - 2502 WP_040182042.1 fimbria/pilus outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49185.49 Da        Isoelectric Point: 7.5358

>T184039 WP_023301940.1 NZ_CP066143:1886010-1887332 [Klebsiella pneumoniae U-0608239]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T184039 NZ_CP087294:c4450314-4450207 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT184039 WP_002904790.1 NZ_CP066143:1885744-1886010 [Klebsiella pneumoniae U-0608239]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT184039 NZ_CP087294:4450371-4450428 [Escherichia coli]
TCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486S9S0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References