Detailed information of TA system TA184000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3638534..3640248
• Replicon: chromosome
• Accession: NZ_CP066125
• Organism: Aeromonas veronii strain 32286

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: JC622_RS17035
• Protein ID: WP_223954276.1
• Coordinates: 3639595..3640248 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: JC622_RS17030
• Protein ID: WP_223954277.1
• Coordinates: 3638534..3639598 (-)
• Length: 355 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JC622_RS17015	3633687..3634625	+	939	WP_021138001.1	restriction endonuclease	-
JC622_RS17020	3634644..3635357	-	714	WP_182954681.1	SprT family zinc-dependent metalloprotease	-
JC622_RS17025	3635357..3638473	-	3117	WP_227095073.1	HsdR family type I site-specific deoxyribonuclease	-
JC622_RS17030	3638534..3639598	-	1065	WP_223954277.1	macro domain-containing protein	Antitoxin
JC622_RS17035	3639595..3640248	-	654	WP_223954276.1	DUF4433 domain-containing protein	Toxin
JC622_RS17040	3640514..3641818	-	1305	WP_227095074.1	restriction endonuclease subunit S	-
JC622_RS17045	3641893..3643035	+	1143	WP_139701073.1	IS4-like element ISAs18 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	katA	3628138..3726862	98724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (18-131)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25346.81 Da
• Isoelectric Point: 6.4041

>T184000 WP_223954276.1 NZ_CP066125:c3640248-3639595 [Aeromonas veronii]
MAYDYSQSLNPEKALIFRIVHRDNLEWILTHGLWAGNGQQQNPHWVHIGLSELIAKRARHPVPLAPYGVLNDYVPFYFTP
FSPMLSNIHAGRGVPQRENDEILILVSSLHRMAELGLPFVFTDSHAYYQWANFYDDLGELGRIDWPLLQRRDFKRDPDDL
SKLERYQAEALIYQHCPIEALQGVVCYNEEIKNSVEQCMNRVGVARPVYVRDGWYFR

Nucleotide

• Length: 654 bp

>T184000 NZ_CP087290:c2064415-2064308 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATAGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 355 a.a.
• Molecular weight: 39606.74 Da
• Isoelectric Point: 7.2908

>AT184000 WP_223954277.1 NZ_CP066125:c3639598-3638534 [Aeromonas veronii]
MIRYTTGNLLDAPAEVLVNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGEVVTGKMFITQTGELLGPRWIVNFPTK
QHWRNPAQMAWIQDGLQDLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGG
VAELTPARALIAELVRRYWFLGMECSLLEIQKLAWFLARVIGRNPQGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQS
DKRIPDSKPFDVIWFNEAKKQELAVYLNTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLMAKEGCAPTVSALREGL
HRWPAGTPWGERKARLFDDRSLQIALTRLQQGLV

Nucleotide

• Length: 1065 bp

>AT184000 NZ_CP087290:2064464-2064530 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC