Detailed information of TA system TA183984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 78922..79510
• Replicon: plasmid unnamed map unlocalized
• Accession: NZ_CP066122
• Organism: Acinetobacter bereziniae strain GD0320

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: I9190_RS21945
• Protein ID: WP_004647749.1
• Coordinates: 79214..79510 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I9190_RS21940
• Protein ID: WP_004969304.1
• Coordinates: 78922..79212 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9190_RS21915 (I9190_21915)	74542..75248	-	707	Protein_75	IS1 family transposase	-
I9190_RS21920 (I9190_21920)	75339..76181	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
I9190_RS21925 (I9190_21925)	76262..76630	+	369	Protein_77	IS1-like element ISAba3 family transposase	-
I9190_RS21930 (I9190_21930)	76897..77106	+	210	WP_004976362.1	hypothetical protein	-
I9190_RS21935 (I9190_21935)	77356..78811	+	1456	Protein_79	SulP family inorganic anion transporter	-
I9190_RS21940 (I9190_21940)	78922..79212	-	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
I9190_RS21945 (I9190_21945)	79214..79510	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9190_RS22510	79726..79851	+	126	WP_004969301.1	hypothetical protein	-
I9190_RS21950 (I9190_21950)	79954..81027	-	1074	WP_043041639.1	hypothetical protein	-
I9190_RS21955 (I9190_21955)	81248..81814	-	567	WP_043041640.1	recombinase family protein	-
I9190_RS21960 (I9190_21960)	82096..82925	+	830	Protein_85	BCCT family transporter	-
I9190_RS21965 (I9190_21965)	82958..83434	-	477	WP_227560729.1	hypothetical protein	-
I9190_RS21970 (I9190_21970)	83515..84219	+	705	WP_001067784.1	IS6-like element IS1006 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / cmlB1 / mph(E) / msr(E) / blaOXA-58	-	1..122921	122921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T183984 WP_004647749.1 NZ_CP066122:c79510-79214 [Acinetobacter bereziniae]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T183984 NZ_CP087288:119906-120304 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT183984 WP_004969304.1 NZ_CP066122:c79212-78922 [Acinetobacter bereziniae]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT183984 NZ_CP087288:119679-119906 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA