183983

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 72068..72656 Replicon plasmid unnamed map unlocalized
Accession NZ_CP066122
Organism Acinetobacter bereziniae strain GD0320

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag I9190_RS21900 Protein ID WP_000438825.1
Coordinates 72369..72656 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag I9190_RS21895 Protein ID WP_004728120.1
Coordinates 72068..72382 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9190_RS21860 (I9190_21860) 68076..69401 + 1326 WP_079393426.1 ISNCY family transposase -
I9190_RS21865 (I9190_21865) 69492..69764 - 273 WP_000369781.1 NadS family protein -
I9190_RS21870 (I9190_21870) 69757..70077 - 321 WP_000897307.1 type II toxin-antitoxin system RelE/ParE family toxin -
I9190_RS21875 (I9190_21875) 70217..70636 + 420 WP_001180106.1 SMI1/KNR4 family protein -
I9190_RS21880 (I9190_21880) 70716..70997 - 282 WP_000985609.1 putative addiction module antidote protein -
I9190_RS21885 (I9190_21885) 70998..71300 - 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
I9190_RS21890 (I9190_21890) 71449..71979 + 531 WP_000172759.1 hypothetical protein -
I9190_RS21895 (I9190_21895) 72068..72382 - 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
I9190_RS21900 (I9190_21900) 72369..72656 - 288 WP_000438825.1 BrnT family toxin Toxin
I9190_RS21905 (I9190_21905) 72886..73491 - 606 WP_000447788.1 LysE family translocator -
I9190_RS21910 (I9190_21910) 73541..74368 - 828 WP_000073658.1 AraC family transcriptional regulator -
I9190_RS21915 (I9190_21915) 74542..75248 - 707 Protein_75 IS1 family transposase -
I9190_RS21920 (I9190_21920) 75339..76181 - 843 WP_002002480.1 carbapenem-hydrolyzing class D beta-lactamase OXA-58 -
I9190_RS21925 (I9190_21925) 76262..76630 + 369 Protein_77 IS1-like element ISAba3 family transposase -
I9190_RS21930 (I9190_21930) 76897..77106 + 210 WP_004976362.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 / cmlB1 / mph(E) / msr(E) / blaOXA-58 - 1..122921 122921


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T183983 WP_000438825.1 NZ_CP066122:c72656-72369 [Acinetobacter bereziniae]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T183983 NZ_CP087288:c110950-110801 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT183983 WP_004728120.1 NZ_CP066122:c72382-72068 [Acinetobacter bereziniae]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT183983 NZ_CP087288:110994-111055 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References