Detailed information of TA system TA183982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 70716..71300
• Replicon: plasmid unnamed map unlocalized
• Accession: NZ_CP066122
• Organism: Acinetobacter bereziniae strain GD0320

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: I9190_RS21885
• Protein ID: WP_000286964.1
• Coordinates: 70998..71300 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I9190_RS21880
• Protein ID: WP_000985609.1
• Coordinates: 70716..70997 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9190_RS21855 (I9190_21855)	66037..67512	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
I9190_RS21860 (I9190_21860)	68076..69401	+	1326	WP_079393426.1	ISNCY family transposase	-
I9190_RS21865 (I9190_21865)	69492..69764	-	273	WP_000369781.1	NadS family protein	-
I9190_RS21870 (I9190_21870)	69757..70077	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I9190_RS21875 (I9190_21875)	70217..70636	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
I9190_RS21880 (I9190_21880)	70716..70997	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
I9190_RS21885 (I9190_21885)	70998..71300	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9190_RS21890 (I9190_21890)	71449..71979	+	531	WP_000172759.1	hypothetical protein	-
I9190_RS21895 (I9190_21895)	72068..72382	-	315	WP_004728120.1	BrnA antitoxin family protein	-
I9190_RS21900 (I9190_21900)	72369..72656	-	288	WP_000438825.1	BrnT family toxin	-
I9190_RS21905 (I9190_21905)	72886..73491	-	606	WP_000447788.1	LysE family translocator	-
I9190_RS21910 (I9190_21910)	73541..74368	-	828	WP_000073658.1	AraC family transcriptional regulator	-
I9190_RS21915 (I9190_21915)	74542..75248	-	707	Protein_75	IS1 family transposase	-
I9190_RS21920 (I9190_21920)	75339..76181	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / cmlB1 / mph(E) / msr(E) / blaOXA-58	-	1..122921	122921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T183982 WP_000286964.1 NZ_CP066122:c71300-70998 [Acinetobacter bereziniae]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T183982 NZ_CP087288:c101772-101485 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT183982 WP_000985609.1 NZ_CP066122:c70997-70716 [Acinetobacter bereziniae]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT183982 NZ_CP087288:c102020-101769 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94