Detailed information of TA system TA183980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 48882..49504
• Replicon: plasmid unnamed map unlocalized
• Accession: NZ_CP066122
• Organism: Acinetobacter bereziniae strain GD0320

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7T4PB98
• Locus tag: I9190_RS21780
• Protein ID: WP_012268403.1
• Coordinates: 48882..49277 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: N9M3S1
• Locus tag: I9190_RS21785
• Protein ID: WP_005155998.1
• Coordinates: 49274..49504 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9190_RS21750 (I9190_21750)	44493..45425	-	933	WP_086374392.1	IS5 family transposase	-
I9190_RS21755 (I9190_21755)	45475..45687	-	213	Protein_43	phosphoglucosamine mutase	-
I9190_RS21760 (I9190_21760)	45774..46589	-	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
I9190_RS22430 (I9190_21770)	48000..48107	+	108	Protein_46	DUF3761 domain-containing protein	-
I9190_RS21775 (I9190_21775)	48325..48789	+	465	WP_004968108.1	hypothetical protein	-
I9190_RS21780 (I9190_21780)	48882..49277	-	396	WP_012268403.1	type II toxin-antitoxin system VapC family toxin	Toxin
I9190_RS21785 (I9190_21785)	49274..49504	-	231	WP_005155998.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
I9190_RS21790 (I9190_21790)	49793..51058	+	1266	WP_020753574.1	CmlA/FloR family chloramphenicol efflux MFS transporter	-
I9190_RS21795 (I9190_21795)	51051..52256	+	1206	WP_020753573.1	ISL3 family transposase	-
I9190_RS21800 (I9190_21800)	52471..53175	+	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
I9190_RS21805 (I9190_21805)	53237..53506	+	270	WP_227505009.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / cmlB1 / mph(E) / msr(E) / blaOXA-58	-	1..122921	122921
	-	inside	IScluster/Tn	aph(6)-Id / aph(3'')-Ib / sul2 / cmlB1	-	35613..53175	17562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14907.18 Da
• Isoelectric Point: 6.2253

>T183980 WP_012268403.1 NZ_CP066122:c49277-48882 [Acinetobacter bereziniae]
MIYLLDTNICIYVINNKPQHVFERFKQYQLGQLAISSITASELAFGVEKSGSERNKQALNKFLSPLEILPYDEQAIWHYA
KLRQDLQSTGKPIGSLDMLIAAHALALDVVLVTNNTKEFERIDGLKLENWV

Nucleotide

• Length: 396 bp

>T183980 NZ_CP066122:c49277-48882 [Acinetobacter bereziniae]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTCATCAACAATAAGCCTCAACACGTTTTTGAACGCTTTAAGCA
ATATCAACTCGGCCAGCTTGCGATTTCAAGTATCACGGCATCTGAGCTGGCTTTCGGTGTAGAGAAGTCTGGATCTGAAC
GCAATAAACAGGCATTAAACAAGTTTTTGTCACCTTTAGAGATTCTTCCTTACGATGAACAAGCAATTTGGCATTACGCC
AAATTACGCCAAGATCTTCAATCCACAGGTAAACCGATTGGCAGCTTAGATATGCTGATCGCTGCTCATGCTTTGGCATT
GGATGTTGTATTAGTAACGAACAATACAAAAGAATTTGAACGGATTGATGGTCTAAAGCTAGAGAATTGGGTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8851.09 Da
• Isoelectric Point: 4.5843

>AT183980 WP_005155998.1 NZ_CP066122:c49504-49274 [Acinetobacter bereziniae]
MEIAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIDNPWDIMLEAINEFEVGFQLERDDQGEQFREEFR

Nucleotide

• Length: 231 bp

>AT183980 NZ_CP066122:c49504-49274 [Acinetobacter bereziniae]
ATGGAAATTGCGAAAGTATTTCAAACTGGTCGCAGTCAAGCGGTTCGCTTGCCTAAAGCCTTTCGCTTTAATGGCACGGA
AGTGGCCATCAAAAGCTTCGGGCGTGGTGTATTACTCATGCCCATCGACAATCCTTGGGATATCATGTTAGAAGCCATCA
ATGAGTTTGAGGTTGGATTCCAGTTAGAGCGAGATGATCAAGGCGAACAGTTCCGTGAGGAGTTCAGATGA

Structures

Toxin

• AlphaFold DB: A0A7T4PB98

Antitoxin

• AlphaFold DB: A0A0A8TT21