Detailed information of TA system TA183974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 21120..21763
• Replicon: plasmid pEho7-3
• Accession: NZ_CP066111
• Organism: Enterobacter hormaechei strain Eho-7

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: GVI68_RS24275
• Protein ID: WP_000754566.1
• Coordinates: 21120..21536 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: GVI68_RS24280
• Protein ID: WP_001261276.1
• Coordinates: 21533..21763 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI68_RS24250 (GVI68_24150)	16232..16996	+	765	WP_197347221.1	IS6-like element IS6100 family transposase	-
GVI68_RS24255 (GVI68_24155)	17489..18073	-	585	WP_001137892.1	macrolide-binding transcriptional repressor MphR(A)	-
GVI68_RS24260 (GVI68_24160)	18073..19311	-	1239	WP_000004159.1	macrolide resistance MFS transporter Mrx(A)	-
GVI68_RS24265 (GVI68_24165)	19308..20213	-	906	WP_000219391.1	Mph(A) family macrolide 2'-phosphotransferase	-
GVI68_RS24270 (GVI68_24170)	20335..21039	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
GVI68_RS24275 (GVI68_24175)	21120..21536	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
GVI68_RS24280 (GVI68_24180)	21533..21763	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GVI68_RS24285 (GVI68_24185)	22352..22645	+	294	WP_020956883.1	hypothetical protein	-
GVI68_RS24290 (GVI68_24190)	22709..23395	+	687	WP_020956882.1	hypothetical protein	-
GVI68_RS24295 (GVI68_24195)	23407..24183	+	777	WP_020956881.1	tyrosine-type recombinase/integrase	-
GVI68_RS24300 (GVI68_24200)	24241..24498	-	258	WP_000764642.1	hypothetical protein	-
GVI68_RS24305 (GVI68_24205)	24627..24731	-	105	WP_032409716.1	hypothetical protein	-
GVI68_RS24310 (GVI68_24210)	25261..26127	+	867	WP_004118283.1	replication initiation protein	-
GVI68_RS24315 (GVI68_24215)	26304..26573	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaVIM-1 / ant(3'')-Ia / qacE / sul1 / mph(A)	-	1..33171	33171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T183974 WP_000754566.1 NZ_CP066111:c21536-21120 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T183974 NZ_CP087287:c2863155-2862904 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT183974 WP_001261276.1 NZ_CP066111:c21763-21533 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT183974 NZ_CP087287:c2862907-2862653 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023