Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 119605..121228 Replicon plasmid pEho7-1
Accession NZ_CP066109
Organism Enterobacter hormaechei strain Eho-7

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag GVI68_RS22565 Protein ID WP_000088044.1
Coordinates 119903..121228 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag GVI68_RS22560 Protein ID WP_012477377.1
Coordinates 119605..119898 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI68_RS22540 (GVI68_22455) 115404..115796 + 393 WP_000341066.1 hypothetical protein -
GVI68_RS22545 (GVI68_22460) 116455..117111 + 657 WP_000462754.1 trypsin-like serine protease -
GVI68_RS22550 (GVI68_22465) 117314..117811 + 498 WP_197347214.1 hypothetical protein -
GVI68_RS22555 (GVI68_22470) 117816..119204 + 1389 WP_000083579.1 hypothetical protein -
GVI68_RS22560 (GVI68_22475) 119605..119898 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
GVI68_RS22565 (GVI68_22480) 119903..121228 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
GVI68_RS22570 (GVI68_22485) 121289..121495 + 207 WP_000134171.1 hypothetical protein -
GVI68_RS22575 (GVI68_22490) 121596..122006 + 411 WP_000985909.1 hypothetical protein -
GVI68_RS22580 (GVI68_22495) 122019..122834 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
GVI68_RS22585 (GVI68_22500) 123087..123512 + 426 WP_000278471.1 hypothetical protein -
GVI68_RS22590 (GVI68_22505) 124061..124369 + 309 WP_001224686.1 hypothetical protein -
GVI68_RS22595 (GVI68_22510) 124385..125242 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / ant(3'')-Ia / catA1 - 1..303895 303895


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T183973 WP_000088044.1 NZ_CP066109:119903-121228 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T183973 NZ_CP087287:c2862907-2862653 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT183973 WP_012477377.1 NZ_CP066109:119605-119898 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT183973 NZ_CP087287:c2863155-2862904 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References