Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7159..7802 Replicon plasmid pEho8-3
Accession NZ_CP066104
Organism Enterobacter hormaechei strain Eho-8

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag GVI69_RS24200 Protein ID WP_000754566.1
Coordinates 7386..7802 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GVI69_RS24195 Protein ID WP_001261276.1
Coordinates 7159..7389 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI69_RS24165 (GVI69_24080) 2795..3661 - 867 WP_004118283.1 replication initiation protein -
GVI69_RS24170 (GVI69_24085) 4191..4295 + 105 WP_032409716.1 hypothetical protein -
GVI69_RS24175 (GVI69_24090) 4424..4681 + 258 WP_000764642.1 hypothetical protein -
GVI69_RS24180 (GVI69_24095) 4739..5515 - 777 WP_020956881.1 tyrosine-type recombinase/integrase -
GVI69_RS24185 (GVI69_24100) 5527..6213 - 687 WP_020956882.1 hypothetical protein -
GVI69_RS24190 (GVI69_24105) 6277..6570 - 294 WP_020956883.1 hypothetical protein -
GVI69_RS24195 (GVI69_24110) 7159..7389 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GVI69_RS24200 (GVI69_24115) 7386..7802 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
GVI69_RS24205 (GVI69_24120) 7883..8587 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
GVI69_RS24210 (GVI69_24125) 8709..9614 + 906 WP_000219391.1 Mph(A) family macrolide 2'-phosphotransferase -
GVI69_RS24215 (GVI69_24130) 9611..10849 + 1239 WP_000004159.1 macrolide resistance MFS transporter Mrx(A) -
GVI69_RS24220 (GVI69_24135) 10849..11433 + 585 WP_001137892.1 macrolide-binding transcriptional repressor MphR(A) -
GVI69_RS24225 (GVI69_24140) 11926..12690 - 765 WP_197347221.1 IS6-like element IS6100 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / sul1 / qacE / ant(3'')-Ia / blaVIM-1 - 1..32990 32990


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T183955 WP_000754566.1 NZ_CP066104:7386-7802 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T183955 NZ_CP087280:c2895882-2895780 [Leclercia adecarboxylata]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT183955 WP_001261276.1 NZ_CP066104:7159-7389 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT183955 NZ_CP087280:2895768-2895918 [Leclercia adecarboxylata]
CTTTCAACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCT
AAAAGTTGGCATTAATGCAGGCTAAGTCGCCTTGCACTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References