Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 118559..120182 Replicon plasmid pEho8-1
Accession NZ_CP066102
Organism Enterobacter hormaechei strain Eho-8

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag GVI69_RS22560 Protein ID WP_000088044.1
Coordinates 118857..120182 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag GVI69_RS22555 Protein ID WP_012477377.1
Coordinates 118559..118852 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI69_RS22535 (GVI69_22465) 114358..114750 + 393 WP_000341066.1 hypothetical protein -
GVI69_RS22540 (GVI69_22470) 115409..116065 + 657 WP_000462754.1 trypsin-like serine protease -
GVI69_RS22545 (GVI69_22475) 116268..116765 + 498 WP_197347214.1 hypothetical protein -
GVI69_RS22550 (GVI69_22480) 116770..118158 + 1389 WP_000083579.1 hypothetical protein -
GVI69_RS22555 (GVI69_22485) 118559..118852 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
GVI69_RS22560 (GVI69_22490) 118857..120182 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
GVI69_RS22565 (GVI69_22495) 120243..120449 + 207 WP_000134171.1 hypothetical protein -
GVI69_RS22570 (GVI69_22500) 120550..120960 + 411 WP_000985909.1 hypothetical protein -
GVI69_RS22575 (GVI69_22505) 120973..121788 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
GVI69_RS22580 (GVI69_22510) 122041..122466 + 426 WP_000278471.1 hypothetical protein -
GVI69_RS22585 (GVI69_22515) 123015..123323 + 309 WP_001224686.1 hypothetical protein -
GVI69_RS22590 (GVI69_22520) 123339..124196 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / ant(3'')-Ia / catA1 - 1..307472 307472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T183954 WP_000088044.1 NZ_CP066102:118857-120182 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T183954 NZ_CP087280:2346462-2346788 [Leclercia adecarboxylata]
ATGGACTACATCCCGGAAGCGGGCGATATCGTCTGGCTGGATTTTGATCCGCAGTCAGGTCATGAACAGGCAGGCCATCG
CCCTGCGCTGGTTATTTCACCCGCCACTTACAATGGACGCACCGGTCTGATGCTCTGCTGTCCAGTCACGTCGCAAATCA
AAAACTACCCCTTCGAAGTTGTCATTAGCGGAGCACGCCGCAATGTCGCCCTCGCCGATCAGATCAAAAGTCTGGACTGG
CGCGCGCGTCACGCAGTGAAAAAGGGAACGGCAACTGCCGAAGAACTGGATGCCGTGAGAATGCGGGCGAAGGTTTTGAT
CGGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT183954 WP_012477377.1 NZ_CP066102:118559-118852 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT183954 NZ_CP087280:2346214-2346462 [Leclercia adecarboxylata]
ATGCCGCAAGTCACCGTCAAAAAATGGGGTAACAGCCCTTCTGTCAGGCTGCCTGTCGCAGTTATGCGCGAGGCTGAATT
GCACGTTGACGATGTGGTGAACGTTAACGTAGACGAAGAAGGACGCATTATTTTGACACCCGTCAGGAAGGACGAACCGA
CGCTGGAATCGCTGCTCGCTGCGATCACCGACGACAATCTGCATCACGAAGTCACCTTTGGAGAACCGCAAGGTAAGGAG
CTGTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References