Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1684942..1685739 Replicon chromosome
Accession NZ_CP066101
Organism Enterobacter hormaechei strain Eho-8

Toxin (Protein)


Gene name KacT Uniprot ID F5RT91
Locus tag GVI69_RS08025 Protein ID WP_006809321.1
Coordinates 1685218..1685739 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag GVI69_RS08020 Protein ID WP_001303459.1
Coordinates 1684942..1685211 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI69_RS07990 (GVI69_07970) 1680564..1681064 + 501 WP_032673548.1 DedA family protein -
GVI69_RS07995 (GVI69_07975) 1681169..1682410 + 1242 WP_006809325.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
GVI69_RS08000 (GVI69_07980) 1682443..1682670 - 228 WP_006809324.1 YccJ family protein -
GVI69_RS08005 (GVI69_07985) 1682689..1683285 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
GVI69_RS08010 (GVI69_07990) 1683676..1683846 + 171 WP_001273664.1 general stress protein -
GVI69_RS08015 (GVI69_07995) 1683963..1684868 + 906 WP_039273532.1 DMT family transporter -
GVI69_RS08020 (GVI69_08000) 1684942..1685211 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
GVI69_RS08025 (GVI69_08005) 1685218..1685739 + 522 WP_006809321.1 GNAT family N-acetyltransferase Toxin
GVI69_RS08030 (GVI69_08010) 1685798..1687120 - 1323 WP_006809320.1 pyrimidine utilization transport protein G -
GVI69_RS08035 (GVI69_08015) 1687142..1687636 - 495 WP_006809319.1 pyrimidine utilization flavin reductase protein F -
GVI69_RS08040 (GVI69_08020) 1687646..1688236 - 591 WP_006809318.1 malonic semialdehyde reductase -
GVI69_RS08045 (GVI69_08025) 1688246..1689046 - 801 WP_006809317.1 pyrimidine utilization protein D -
GVI69_RS08050 (GVI69_08030) 1689054..1689440 - 387 WP_006809316.1 pyrimidine utilization protein C -
GVI69_RS08055 (GVI69_08035) 1689452..1690141 - 690 WP_006809315.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19494.28 Da        Isoelectric Point: 7.9346

>T183942 WP_006809321.1 NZ_CP066101:1685218-1685739 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGNFDCGEPSLNAFLTDHLVRQHSGRILRGYLLKERDHPRILGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQV

Download         Length: 522 bp

>T183942 NZ_CP087272:c5355742-5355359 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT183942 WP_001303459.1 NZ_CP066101:1684942-1685211 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT183942 NZ_CP087272:c5355984-5355742 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1R089

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References