Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1117522..1118142 Replicon chromosome
Accession NZ_CP066101
Organism Enterobacter hormaechei strain Eho-8

Toxin (Protein)


Gene name Hha Uniprot ID F5RUW8
Locus tag GVI69_RS05360 Protein ID WP_001291436.1
Coordinates 1117522..1117740 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag GVI69_RS05365 Protein ID WP_006809850.1
Coordinates 1117768..1118142 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI69_RS05325 (GVI69_05320) 1112747..1113553 - 807 WP_032673625.1 nickel/cobalt efflux protein RcnA -
GVI69_RS05330 (GVI69_05325) 1113688..1113960 + 273 WP_006809855.1 metal-sensing transcriptional repressor -
GVI69_RS05335 (GVI69_05330) 1113953..1115521 - 1569 WP_237892389.1 EAL domain-containing protein -
GVI69_RS05340 (GVI69_05335) 1115739..1115999 + 261 WP_006809853.1 type B 50S ribosomal protein L31 -
GVI69_RS05345 (GVI69_05340) 1116002..1116142 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
GVI69_RS05350 (GVI69_05345) 1116178..1116645 - 468 WP_006809852.1 YlaC family protein -
GVI69_RS05355 (GVI69_05350) 1116762..1117313 - 552 WP_006809851.1 maltose O-acetyltransferase -
GVI69_RS05360 (GVI69_05355) 1117522..1117740 - 219 WP_001291436.1 HHA domain-containing protein Toxin
GVI69_RS05365 (GVI69_05360) 1117768..1118142 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
GVI69_RS05370 (GVI69_05365) 1118651..1121797 - 3147 WP_006809849.1 multidrug efflux RND transporter permease subunit AcrB -
GVI69_RS05375 (GVI69_05370) 1121820..1123013 - 1194 WP_006809848.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8597.97 Da        Isoelectric Point: 8.9008

>T183940 WP_001291436.1 NZ_CP066101:c1117740-1117522 [Enterobacter hormaechei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T183940 NZ_CP087272:c4875552-4875268 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT183940 WP_006809850.1 NZ_CP066101:c1118142-1117768 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT183940 NZ_CP087272:c4875784-4875542 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1VNQ1

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References