Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 936558..937237 Replicon chromosome
Accession NZ_CP066101
Organism Enterobacter hormaechei strain Eho-8

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2J0PMI2
Locus tag GVI69_RS04455 Protein ID WP_039273191.1
Coordinates 936558..936899 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2J0PNI1
Locus tag GVI69_RS04460 Protein ID WP_039273193.1
Coordinates 936920..937237 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI69_RS04435 (GVI69_04430) 932550..933653 + 1104 WP_006809995.1 glutamate 5-kinase -
GVI69_RS04440 (GVI69_04435) 933665..934918 + 1254 WP_006809994.1 glutamate-5-semialdehyde dehydrogenase -
GVI69_RS04450 (GVI69_04445) 935512..936480 + 969 WP_000654811.1 IS5 family transposase -
GVI69_RS04455 (GVI69_04450) 936558..936899 - 342 WP_039273191.1 TA system toxin CbtA family protein Toxin
GVI69_RS04460 (GVI69_04455) 936920..937237 - 318 WP_039273193.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GVI69_RS04465 (GVI69_04460) 937257..937478 - 222 WP_039273195.1 DUF987 domain-containing protein -
GVI69_RS04470 (GVI69_04465) 937487..937963 - 477 WP_039273197.1 RadC family protein -
GVI69_RS04475 (GVI69_04470) 937979..938440 - 462 Protein_861 antirestriction protein -
GVI69_RS04480 (GVI69_04475) 938531..938703 - 173 Protein_862 DUF905 family protein -
GVI69_RS04485 (GVI69_04480) 939184..941181 + 1998 WP_039274705.1 choline BCCT transporter BetT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 936558..982801 46243
- flank IS/Tn - - 935557..936480 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12851.82 Da        Isoelectric Point: 9.6545

>T183939 WP_039273191.1 NZ_CP066101:c936899-936558 [Enterobacter hormaechei]
MKPLPATTSRAAKPCLSPVAVWQILLTHLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDRRGF
SRQEQSPYLRAVDILRARQVTGLLRQSRNNVVR

Download         Length: 342 bp

>T183939 NZ_CP087272:c4752227-4751694 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11890.58 Da        Isoelectric Point: 7.7498

>AT183939 WP_039273193.1 NZ_CP066101:c937237-936920 [Enterobacter hormaechei]
MSNITWGLQRNITPRLGARLVQDRNRMHYLADRASITGKFSDAECLKLDVAFPHFISQMESMLTTGELNPRHPHCIALYH
NGFTCEADTLGSCGYVYIAVYPSQR

Download         Length: 318 bp

>AT183939 NZ_CP087272:c4752504-4752238 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PMI2

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PNI1

References