Detailed information of TA system TA183938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 66610..67266
• Replicon: chromosome
• Accession: NZ_CP066101
• Organism: Enterobacter hormaechei strain Eho-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GVI69_RS00340
• Protein ID: WP_006812483.1
• Coordinates: 66874..67266 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F5S3Q5
• Locus tag: GVI69_RS00335
• Protein ID: WP_006812484.1
• Coordinates: 66610..66870 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI69_RS00305 (GVI69_00305)	61811..62125	-	315	WP_006812490.1	PTS sugar transporter subunit IIB	-
GVI69_RS00310 (GVI69_00310)	62419..63369	+	951	WP_006812489.1	LacI family DNA-binding transcriptional regulator	-
GVI69_RS00315 (GVI69_00315)	63417..63713	-	297	WP_003861030.1	YicS family protein	-
GVI69_RS00320 (GVI69_00320)	63851..64270	+	420	WP_006812487.1	GNAT family N-acetyltransferase	-
GVI69_RS00325 (GVI69_00325)	64267..64914	-	648	WP_006812486.1	TetR/AcrR family transcriptional regulator	-
GVI69_RS00330 (GVI69_00330)	64993..66492	+	1500	WP_039272833.1	MDR family MFS transporter	-
GVI69_RS00335 (GVI69_00335)	66610..66870	+	261	WP_006812484.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GVI69_RS00340 (GVI69_00340)	66874..67266	+	393	WP_006812483.1	type II toxin-antitoxin system VapC family toxin	Toxin
GVI69_RS00345 (GVI69_00345)	67335..67808	-	474	WP_032674176.1	hypothetical protein	-
GVI69_RS00350 (GVI69_00350)	68190..68990	+	801	WP_032674175.1	lipoprotein NlpA	-
GVI69_RS00355 (GVI69_00355)	69024..69704	-	681	WP_000288876.1	EAL domain-containing protein	-
GVI69_RS00360 (GVI69_00360)	69780..70400	-	621	WP_006812480.1	LuxR C-terminal-related transcriptional regulator	-
GVI69_RS00365 (GVI69_00365)	71106..71606	+	501	WP_237892367.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14587.75 Da
• Isoelectric Point: 5.8985

>T183938 WP_006812483.1 NZ_CP066101:66874-67266 [Enterobacter hormaechei]
MLHMLDTNIVSHLVRQHPEVVNRYSQITPEKMCISSVTEAELLYGVAKKQNNKLHETIMEFLKTITICAWDSEAAATYGE
LRAAMEKKGKVMGDLDQLIAAHAISRGTTIVTNDHAFGMVQDLTVEDWTQ

Nucleotide

• Length: 393 bp

>T183938 NZ_CP087272:4150146-4150364 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10169.45 Da
• Isoelectric Point: 9.7289

>AT183938 WP_006812484.1 NZ_CP066101:66610-66870 [Enterobacter hormaechei]
MERTAKLFKKGRNQAVVLPAEFAFETESVYIRRDDEGNVVLTARSEKERHRDNFLRMLRQTHVPDTFLSKEERNQSYTTR
NPLEGL

Nucleotide

• Length: 261 bp

>AT183938 NZ_CP087272:4149745-4150119 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7T1QFB1