Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 117003..118626 Replicon plasmid pEhoE1-1
Accession NZ_CP066095
Organism Enterobacter hormaechei strain Eho-E1

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag I9464_RS25435 Protein ID WP_000088044.1
Coordinates 117301..118626 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag I9464_RS25430 Protein ID WP_012477377.1
Coordinates 117003..117296 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9464_RS25410 (I9464_25375) 112802..113194 + 393 WP_000341066.1 hypothetical protein -
I9464_RS25415 (I9464_25380) 113853..114509 + 657 WP_000462754.1 trypsin-like serine protease -
I9464_RS25420 (I9464_25385) 114712..115209 + 498 WP_000340139.1 membrane protein -
I9464_RS25425 (I9464_25390) 115214..116602 + 1389 WP_000083579.1 hypothetical protein -
I9464_RS25430 (I9464_25395) 117003..117296 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
I9464_RS25435 (I9464_25400) 117301..118626 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
I9464_RS25440 (I9464_25405) 118687..118893 + 207 WP_000134171.1 hypothetical protein -
I9464_RS25445 (I9464_25410) 118994..119404 + 411 WP_000985909.1 hypothetical protein -
I9464_RS25450 (I9464_25415) 119417..120232 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
I9464_RS25455 (I9464_25420) 120485..120910 + 426 WP_000278471.1 hypothetical protein -
I9464_RS25460 (I9464_25425) 121459..121767 + 309 WP_001224686.1 hypothetical protein -
I9464_RS25465 (I9464_25430) 121783..122640 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaACC-1 / sul1 / aph(6)-Id / aph(3'')-Ib / mcr-9 / qacE - 1..331870 331870


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T183936 WP_000088044.1 NZ_CP066095:117301-118626 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T183936 NZ_CP087272:1982139-1982241 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT183936 WP_012477377.1 NZ_CP066095:117003-117296 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT183936 NZ_CP087272:c1982248-1982103 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References