Detailed information of TA system TA183935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 5027859..5028475
• Replicon: chromosome
• Accession: NZ_CP066094
• Organism: Enterobacter hormaechei strain Eho-E1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I9464_RS24470
• Protein ID: WP_015569913.1
• Coordinates: 5027859..5028230 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: I9464_RS24475
• Protein ID: WP_015569912.1
• Coordinates: 5028233..5028475 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9464_RS24455 (I9464_24420)	5025359..5026261	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
I9464_RS24460 (I9464_24425)	5026258..5026893	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
I9464_RS24465 (I9464_24430)	5026890..5027819	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
I9464_RS24470 (I9464_24435)	5027859..5028230	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
I9464_RS24475 (I9464_24440)	5028233..5028475	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
I9464_RS24480 (I9464_24445)	5028674..5029594	+	921	WP_015569911.1	alpha/beta hydrolase	-
I9464_RS24485 (I9464_24450)	5029603..5030544	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
I9464_RS24490 (I9464_24455)	5030589..5031026	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
I9464_RS24495 (I9464_24460)	5031023..5031904	-	882	WP_003861949.1	virulence factor BrkB family protein	-
I9464_RS24500 (I9464_24465)	5031898..5032497	-	600	WP_003861947.1	glucose-1-phosphatase	-
I9464_RS24505 (I9464_24470)	5032616..5033416	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T183935 WP_015569913.1 NZ_CP066094:c5028230-5027859 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T183935 NZ_CP087272:869842-870252 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT183935 WP_015569912.1 NZ_CP066094:c5028475-5028233 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT183935 NZ_CP087272:869595-869861 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3