Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3080202..3080807 Replicon chromosome
Accession NZ_CP066094
Organism Enterobacter hormaechei strain Eho-E1

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag I9464_RS14870 Protein ID WP_012906750.1
Coordinates 3080202..3080381 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A822X3T2
Locus tag I9464_RS14875 Protein ID WP_032619507.1
Coordinates 3080403..3080807 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9464_RS14840 (I9464_14815) 3075531..3076247 + 717 WP_032619512.1 ABC transporter ATP-binding protein -
I9464_RS14845 (I9464_14820) 3076413..3076883 + 471 WP_017384432.1 hypothetical protein -
I9464_RS14850 (I9464_14825) 3076889..3077815 - 927 WP_032619511.1 LysR substrate-binding domain-containing protein -
I9464_RS14855 (I9464_14830) 3077845..3078168 - 324 WP_017384434.1 hypothetical protein -
I9464_RS14860 3078171..3078518 - 348 WP_230199855.1 DUF4225 domain-containing protein -
I9464_RS14865 3078585..3078926 - 342 WP_223862348.1 DUF4225 domain-containing protein -
I9464_RS14870 (I9464_14840) 3080202..3080381 + 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
I9464_RS14875 (I9464_14845) 3080403..3080807 + 405 WP_032619507.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I9464_RS14880 (I9464_14850) 3080848..3081378 - 531 WP_176452122.1 glycosyltransferase family 9 protein -
I9464_RS14885 (I9464_14855) 3081402..3082373 - 972 WP_197386120.1 IS481 family transposase -
I9464_RS14890 (I9464_14860) 3082465..3083007 - 543 WP_237902849.1 hypothetical protein -
I9464_RS14895 (I9464_14865) 3083084..3083662 - 579 WP_032619505.1 tail fiber assembly protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fliA 3075543..3186306 110763
- flank IS/Tn - - 3081402..3082373 971


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T183931 WP_012906750.1 NZ_CP066094:3080202-3080381 [Enterobacter hormaechei]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T183931 NZ_CP087271:3182-3490 [Aeromonas veronii]
ATGCAGTTCACGATCTATCGCAACAAGGGCAATGCCCGCGATTATCCCTACTTGCTGAATGTGCAAAGCGACATCATCGA
TGAACTGAACACTAGGATGGTGATCCCGTTGGTACCGCTGGCGGCCTTCATTGGGCGGCCAGCGCAACGACTAAACCCCG
TTATTTCCGTCGAAGGAAAAAAGCATCTGGTGTTGACCCAAGAAATGGCGTCGGTTCGGTTGGGGCAGCTTGGAGAGGAA
GTCACTGACGTGCAGGAGTCTCGTCAGCTCATCAAGGACTCAATAGATTTCCTACTGGATGGAGTGTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14676.53 Da        Isoelectric Point: 4.2136

>AT183931 WP_032619507.1 NZ_CP066094:3080403-3080807 [Enterobacter hormaechei]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGEDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVINQR

Download         Length: 405 bp

>AT183931 NZ_CP087271:2937-3182 [Aeromonas veronii]
ATGGTCACCGCAACAATGCGTATACGCAGCAAGAGAACGGTCAGCGTCACGCTGGAGCCCAGCTTGCTGGAACGAGCCAG
AGCGGCGGGGATCAACCTGTCTGCTACCCTAGCCACCGCGCTACAGCATGAAATCAGCGGCTCAGAGGCCGAGCGTTGGA
AGCGAGATAACAGGGAGGCAATCCAAACCCTTAATCGGATGGTCGAAGAGCGCGGCCTGTTTTCTGACGATCACAGGACG
TTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A822X3T2

References