Detailed information of TA system TA183915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1136..1610
• Replicon: plasmid unnamed1
• Accession: NZ_CP066090
• Organism: Citrobacter koseri strain FDAARGOS_1029

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: I6H86_RS21920
• Protein ID: WP_004197762.1
• Coordinates: 1344..1610 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: I6H86_RS21915
• Protein ID: WP_000879771.1
• Coordinates: 1136..1360 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H86_RS21905	118..249	+	132	WP_198478784.1	mbkB	-
I6H86_RS21910	256..468	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
I6H86_RS21915	1136..1360	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
I6H86_RS21920	1344..1610	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H86_RS21925	1680..2030	-	351	WP_004197756.1	hypothetical protein	-
I6H86_RS21930	2096..2641	-	546	WP_004197758.1	hypothetical protein	-
I6H86_RS21935	2667..3050	-	384	WP_004198370.1	hypothetical protein	-
I6H86_RS21940	4153..4329	+	177	WP_004197757.1	hypothetical protein	-
I6H86_RS21945	4319..4663	+	345	WP_004197764.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5601	5601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T183915 WP_004197762.1 NZ_CP066090:1344-1610 [Citrobacter koseri]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T183915 NZ_CP087226:c1019209-1018904 [Dickeya fangzhongdai]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCCAAGCGACGCATGGTCATTCCGCTTATCGAATCGCATCACCTGTCTGAGAAAGTGAATAAGATGTTGTTCCCTCTGC
TTCGCATCGATGGCGAAGATTATCGGTTGATGACAACTGAACTATCGAGCGTTCCTGTTGAGGTTATTGGTGAAGAAATC
GCGGATCTTGGCGACTACGCTGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT183915 WP_000879771.1 NZ_CP066090:1136-1360 [Citrobacter koseri]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT183915 NZ_CP087226:c1019430-1019212 [Dickeya fangzhongdai]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCCGCCGGGGTCAATATTTCCGGGCT
GGTGAATGAGGCCATTGGCAAAGAAGCCCGCCGCATCAGGGCTGAAGAGTGGAAGAAGGAGAATCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAGAATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5