Detailed information of TA system TA183913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2687909..2688525
• Replicon: chromosome
• Accession: NZ_CP066089
• Organism: Citrobacter koseri strain FDAARGOS_1029

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A8AL49
• Locus tag: I6H86_RS12530
• Protein ID: WP_012133919.1
• Coordinates: 2687909..2688283 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: I6H86_RS12535
• Protein ID: WP_160880417.1
• Coordinates: 2688283..2688525 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H86_RS12515	2685412..2686314	+	903	WP_012133916.1	formate dehydrogenase O subunit beta	-
I6H86_RS12520	2686311..2686946	+	636	WP_012133917.1	formate dehydrogenase cytochrome b556 subunit	-
I6H86_RS12525	2686943..2687872	+	930	WP_012133918.1	formate dehydrogenase accessory protein FdhE	-
I6H86_RS12530	2687909..2688283	-	375	WP_012133919.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6H86_RS12535	2688283..2688525	-	243	WP_160880417.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6H86_RS12540	2688761..2689702	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
I6H86_RS12545	2689747..2690184	-	438	WP_012133923.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6H86_RS12550	2690181..2691053	-	873	WP_012133924.1	virulence factor BrkB family protein	-
I6H86_RS12555	2691047..2691646	-	600	WP_024130671.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13741.00 Da
• Isoelectric Point: 9.8061

>T183913 WP_012133919.1 NZ_CP066089:c2688283-2687909 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRGEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T183913 NZ_CP087226:575884-576375 [Dickeya fangzhongdai]
ATGATTTCCGCCCCTGAGCCACTCCGCGCCGAACATGTACTATCTTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GTTGAAACAACGGGCGATGAAAAATCAGGTCACTGGAGCATCACGAACTTTCGTTAGTTGCAATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGTGTTGTGGCAACGAATGCCGCTCCCGGACGTTTTCGCCGCAATATGCCCGACCCTATT
CCGGTCGTGGTACTGGGTCGTCTGGCGGTAGATAAATCATTACATGGTCAAGGCGTTGGCCGAGCGCTGGTGCGCGATGC
CGGGCTGCGGGTGATTCAGGTGGCGGATACTATCGGTATTCGCGGGATGTTGGTTCATGCGCTGTCTGATGAAGCGCGGG
AGTTTTACCTGCGGGTGGGATTTGAGCCTTCTCCGATTGATCCGATGATGCTTATGGTGACGTTGGGGGATTTGGTGGGA
TCTGTAATTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9396.62 Da
• Isoelectric Point: 4.1961

>AT183913 WP_160880417.1 NZ_CP066089:c2688525-2688283 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW

Nucleotide

• Length: 243 bp

>AT183913 NZ_CP087226:575594-575887 [Dickeya fangzhongdai]
ATGCCAGCGACGAACAGCGTTTCTGTTAAACGAGAAACATTGAATTTACGCATCAAGCCTGCCGAACGCGATCTGATTGA
TCGTGCTGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTCCTTGAGGCTGCGCGTGCCGCCGCAGAGGAAGCGTTGA
TAGATCAGCGCATCATCATGGCAGATCCCGATGCTTATCAGGAATTCCTTGCCCGTCTAGATCAGGCTCCTTCACCGAAT
GCCGCTCTGCGTAAAACCATGCAAACACCTGCACCGTGGGAACAGAAAAAATGA

Structures

Toxin

• AlphaFold DB: A8AL49