Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1579793..1580413 Replicon chromosome
Accession NZ_CP066089
Organism Citrobacter koseri strain FDAARGOS_1029

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I6H86_RS07475 Protein ID WP_002892050.1
Coordinates 1580195..1580413 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag I6H86_RS07470 Protein ID WP_012133514.1
Coordinates 1579793..1580167 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H86_RS07460 1574929..1576122 + 1194 WP_012133512.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6H86_RS07465 1576145..1579291 + 3147 WP_012133513.1 efflux RND transporter permease AcrB -
I6H86_RS07470 1579793..1580167 + 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
I6H86_RS07475 1580195..1580413 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I6H86_RS07480 1580597..1581148 + 552 WP_012133515.1 maltose O-acetyltransferase -
I6H86_RS07485 1581262..1581732 + 471 WP_024130587.1 membrane protein -
I6H86_RS07490 1581810..1581950 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
I6H86_RS07495 1581952..1582212 - 261 WP_012133519.1 type B 50S ribosomal protein L31 -
I6H86_RS07500 1582438..1583988 + 1551 WP_024130588.1 EAL domain-containing protein -
I6H86_RS07505 1584028..1584381 - 354 WP_012133521.1 DUF1428 family protein -
I6H86_RS07510 1584461..1585075 - 615 WP_012133522.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1579793..1587837 8044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T183911 WP_002892050.1 NZ_CP066089:1580195-1580413 [Citrobacter koseri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T183911 NZ_CP087205:c6857526-6857248 [Pseudomonas sp. B21-053]
ATGATCGTTAGCTTTAAGTGTTCTGAGACCGAGTACCTGTTTCGCAACGGTAAGACCCGTATGTGGTCAGCCCTTCTGAA
TGTCGTGGAACGAAAGCTGACGATGCTTGATGCTGCTGCCGTTCTGATGGACCTTCGATCACCGCCGGGCAATCGCTTGG
AGGCGTTGGAGGGGAGTCGAAAAGGTCAGTACAGCATCCGAATCAACGCTCAATGGCGAATTTGCTTTGTATGGGGTCCT
AGCGGACCAGAGAATGTCGAAATCGTCGATTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT183911 WP_012133514.1 NZ_CP066089:1579793-1580167 [Citrobacter koseri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT183911 NZ_CP087205:c6857239-6856934 [Pseudomonas sp. B21-053]
ATGACTAAAAACGGTATGCGCCCGGTCCACCCCGGCGAGATCCTCAAAGAGGAATACCTTGAGCCACTGAGCCTTACAGC
GGCGGCTTTGGCCAGAGCATTGAGCGTTTCGACACCGACGGTGAATGACATCGTGCTTCAGCGTCGAGGCGTCAGTGCTG
ATATGGCTCTCAGATTATCTATCTGCCTGGACACGACTCCCGAGTTTTGGCTGAACCTGCAATCGACTTACGATTTGCGT
AAAGCCGAAATTGAACGAGGGGCTGCGATTCGGGATCAGGTTGAGCGTCTTGCGCACTGCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References