Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 409646..410222 Replicon chromosome
Accession NZ_CP066085
Organism Escherichia fergusonii strain FDAARGOS_1032

Toxin (Protein)


Gene name shpA Uniprot ID A0A7W3EF74
Locus tag I6H89_RS02065 Protein ID WP_024256534.1
Coordinates 409935..410222 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7U9DAW4
Locus tag I6H89_RS02060 Protein ID WP_000063150.1
Coordinates 409646..409948 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H89_RS02040 405460..405972 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
I6H89_RS02045 406242..408392 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
I6H89_RS02050 408442..408645 + 204 WP_000467859.1 YbdD/YjiX family protein -
I6H89_RS02055 408656..409612 + 957 WP_001297640.1 GTPase -
I6H89_RS02060 409646..409948 - 303 WP_000063150.1 BrnA antitoxin family protein Antitoxin
I6H89_RS02065 409935..410222 - 288 WP_024256534.1 BrnT family toxin Toxin
I6H89_RS02070 410378..411112 - 735 WP_000208601.1 Fic family protein -
I6H89_RS02075 411375..414887 + 3513 WP_000981386.1 type I restriction-modification system endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11241.74 Da        Isoelectric Point: 7.4020

>T183874 WP_024256534.1 NZ_CP066085:c410222-409935 [Escherichia fergusonii]
MPMEFEWDANKAKSNRMKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T183874 NZ_CP087198:2443197-2443478 [Pseudomonas sp. B21-010]
ATGACCTATGAGTTGGAGTTTTCCGATAAAGCATGGAAGGAATGGCAAAAGCTGGGAGCAACACTCAAGGAGCAATTCAA
GAAAAAGCTTGAGGAGCGACTGGTAAATCCGCATGTCCAGGCAGACCGACTGCACGGGTTGGGCAATGCCTATAAAATCA
AGTTGCGTAACGCCGGCTATCGATTGGTTTATCGGGTAGTAGATGACTTGCTGGTTGTTACGGTGATCGCAGTAGGTAAA
CGCGAGCGCGGCCAAGTGTATGACGAGGCTGCCAAGCGGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11532.03 Da        Isoelectric Point: 9.4646

>AT183874 WP_000063150.1 NZ_CP066085:c409948-409646 [Escherichia fergusonii]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT183874 NZ_CP087198:2442959-2443207 [Pseudomonas sp. B21-010]
ATGACACATATCGTGCTTTCTCAGGTTGTAGCCAGCATTTCCGAGCTTAAGAAAAATCCGATGGGCACTGTCGCTGCCGG
GGGAGGATTATCGGTAGCGATCCTCAACCGCAACGAGCCGGCTTTTTATTGCGTCCCCGCTCGGGAATATGAAGCCATGA
TGGATCGGCTTGAGGACATGGAGTTGGTCGCTCTCTGCCGCGAGCGTGAAAATGATCCAACGATCAAGGTTTCTCTTGAT
GACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3EF74

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAW4

References