Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2024853..2025504 Replicon chromosome
Accession NZ_CP066071
Organism Providencia stuartii strain FDAARGOS_1040

Toxin (Protein)


Gene name Hha Uniprot ID K8W3H1
Locus tag I6H97_RS09115 Protein ID WP_004927064.1
Coordinates 2024853..2025056 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID B2Q721
Locus tag I6H97_RS09120 Protein ID WP_004927067.1
Coordinates 2025136..2025504 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H97_RS09090 2020731..2021069 + 339 WP_004927053.1 P-II family nitrogen regulator -
I6H97_RS09095 2021080..2022363 + 1284 WP_014656764.1 ammonium transporter AmtB -
I6H97_RS09100 2022600..2023466 - 867 WP_004927058.1 acyl-CoA thioesterase II -
I6H97_RS09105 2023789..2024247 + 459 WP_004927062.1 YbaY family lipoprotein -
I6H97_RS09115 2024853..2025056 - 204 WP_004927064.1 hemolysin expression modulator Hha Toxin
I6H97_RS09120 2025136..2025504 - 369 WP_004927067.1 Hha toxicity modulator TomB Antitoxin
I6H97_RS09125 2026072..2027409 - 1338 WP_004927077.1 murein transglycosylase D -
I6H97_RS09130 2027488..2028243 - 756 WP_040132835.1 hydroxyacylglutathione hydrolase -
I6H97_RS09135 2028277..2029002 + 726 WP_040132836.1 methyltransferase domain-containing protein -
I6H97_RS09140 2029081..2029551 - 471 WP_014656762.1 ribonuclease HI -
I6H97_RS09145 2029606..2030367 + 762 WP_004927092.1 DNA polymerase III subunit epsilon -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8075.37 Da        Isoelectric Point: 6.9770

>T183862 WP_004927064.1 NZ_CP066071:c2025056-2024853 [Providencia stuartii]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSEDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Download         Length: 204 bp

>T183862 NZ_CP087195:4625034-4625342 [Pseudomonas brassicacearum]
ATGAGCCAACACCTGACGACATTTGACATGGCGGCACTACTCGATAGCGACGAAGCCATCAGTGAATACCTTTCCCAAGT
GCTGGCTGATGGTGATAGCGAGGAGTTTCTCCGAGCGATCGGCTATGTCGCCAAGGCCCGAGGCATGGCACAGATCGCTA
AGGACTCTGGCATGGGACGAGAAAGCTTGTACAAGGCGTTCGCACCCGGATCAAAGCCGAGATTTGATACCGTACTCAAG
GTCATTCATGCGCTAGGCATTGATCTTCATGCGCAGCCTGGGCATGCAGCTAATCATTCACCTGCCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14149.01 Da        Isoelectric Point: 4.2591

>AT183862 WP_004927067.1 NZ_CP066071:c2025504-2025136 [Providencia stuartii]
MDEYSPKNYDISELKYLCNSLNREAMLSLQKTNTHWVNDLSSPQSARLNELIEHIAAFVWQFKIKYPKENLVISLVEEYL
DETYDLFGSPVITLSEIIDWQSMNQNLVSVLDDDLKCPASKT

Download         Length: 369 bp

>AT183862 NZ_CP087195:4624741-4625031 [Pseudomonas brassicacearum]
ATTTACCGCATACAGCAAACGCAGACGTTCACTGCTTGGCATTCATCGGTCCGTGATCTGCGAGCAAAAAACGCTATTGC
CCGCCGCATTGATCGCGCCGCAGCAGGTAACCTGGGCGATGTGAAACCTGTCGGTGATGGTATTTCAGAGTTGCGTGTGG
ATGTAGGCGCTGGCTACCGGGACTATTTCACCATGCGTGGTGGATTGGTCATCGTGCTACTGGCGGGTGGTGACAAGTCT
TCACAGAGTGCTGATATCCGGCGAGCGAAGAAATTGGCAAAGGAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8W3H1

Antitoxin

Source ID Structure
AlphaFold DB A0A140NKN2

References