Detailed information of TA system TA183842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 2056742..2057350
• Replicon: chromosome
• Accession: NZ_CP066058
• Organism: Alcaligenes faecalis strain FDAARGOS_1024

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3G6HLB3
• Locus tag: I6H81_RS09355
• Protein ID: WP_042487248.1
• Coordinates: 2057060..2057350 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: I6H81_RS09350
• Protein ID: WP_042487247.1
• Coordinates: 2056742..2057047 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H81_RS09325	2052246..2053061	-	816	WP_042487233.1	3-(cis-5,6-dihydroxycyclohexa-1, 3-dien-1-yl)propanoate dehydrogenase	-
I6H81_RS09330	2053058..2053378	-	321	WP_026484793.1	bifunctional 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit	-
I6H81_RS09335	2053407..2053955	-	549	WP_042487236.1	3-phenylpropionate/cinnamic acid dioxygenase subunit beta	-
I6H81_RS09340	2053952..2055310	-	1359	WP_042487239.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
I6H81_RS09345	2055697..2056650	+	954	WP_042487243.1	LysR family transcriptional regulator	-
I6H81_RS09350	2056742..2057047	-	306	WP_042487247.1	putative addiction module antidote protein	Antitoxin
I6H81_RS09355	2057060..2057350	-	291	WP_042487248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H81_RS09360	2057400..2058788	-	1389	WP_165589484.1	ATP-dependent RNA helicase DbpA	-
I6H81_RS09365	2058846..2059619	-	774	WP_042487251.1	IclR family transcriptional regulator	-
I6H81_RS09370	2059936..2061411	+	1476	WP_042487254.1	NAD-dependent succinate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-95)

Antitoxin

• Uncharacterized protein HI1420 (5-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11029.53 Da
• Isoelectric Point: 6.7185

>T183842 WP_042487248.1 NZ_CP066058:c2057350-2057060 [Alcaligenes faecalis]
MKTIHTIDTFDHWFGGLRDKQAARRIQVRIDRAEDGNFGDCEPVGEGVPEMRIHYGPGYRVYFVQRELEIVILLADGNKS
TQNKDIKTALEIARQL

Nucleotide

• Length: 291 bp

>T183842 NZ_CP087190:c946988-946698 [Pseudomonas sp. B21-023]
ATGACCTATAACCTCGATTTCGATGCGCGCGCACTCAAGGAATGGCAGAAGCTCGGCGATACCATTCGCCAACAGCTCAA
GAAGAAGCTGGCAACCGTATTGCTCAACCCGAGGATCGAAGCGAACCGTCTGCACGGTTTCCCGGACTGCTACAAGATCA
AGCTGCGCAGCAGCGGATATCGATTGGTCTATCAGGTCATCGATCATCAAATCGTGGTGTTTGTGGTCGCCGTGGATAAA
CGTGAGCGAGATCAGGTTTACCGCAAAGCGGCCGAACGATTGACCGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10940.59 Da
• Isoelectric Point: 5.6998

>AT183842 WP_042487247.1 NZ_CP066058:c2057047-2056742 [Alcaligenes faecalis]
MGTIKLRKWDSAEYLQTEEDMVLYLEACMEEAGDDAAFIAKALGNIARAKGMSQLAKETGLGRESLYKALSGEGNPSFGT
ILKVMHALGIKLQPQLAPHNS

Nucleotide

• Length: 306 bp

>AT183842 NZ_CP087190:c947217-946978 [Pseudomonas sp. B21-023]
ATGGCGTCCATCAATGTACGTATCGACGACGACCTCAAGGTGCGCGCCTACCGTGAGCTGGAAAAGCTGGGTGTAACCCC
CTCCGAGCTGATGCGCCAAGCGCTGCAATATGTTGCTGAGCGAGGGCAACTGCCTTTTAAACCCGTGTTACTGACCGAAG
AGGACGAAGCACTGCTCGCCACGGTACGCGAGCGTCTGGCCGCCCCGCAAAGGGTGAGGGTAACGCTGGATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A3G6HLB3