Detailed information of TA system TA183835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 54351..54873
• Replicon: plasmid unnamed1
• Accession: NZ_CP066051
• Organism: Brucella anthropi strain FDAARGOS_1039

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A6X7M9
• Locus tag: I6H96_RS14480
• Protein ID: WP_011982866.1
• Coordinates: 54351..54638 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A6X7N0
• Locus tag: I6H96_RS14485
• Protein ID: WP_011982865.1
• Coordinates: 54628..54873 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H96_RS14455	49474..49815	+	342	WP_011982871.1	DUF736 family protein	-
I6H96_RS14460	49936..50136	+	201	WP_011982870.1	antitoxin VbhA family protein	-
I6H96_RS14465	50652..52055	+	1404	WP_011982869.1	group II intron reverse transcriptase/maturase	-
I6H96_RS14470	52131..53180	+	1050	WP_011982868.1	Fic family protein	-
I6H96_RS14475	53394..54281	+	888	WP_011982867.1	DUF1738 domain-containing protein	-
I6H96_RS14480	54351..54638	-	288	WP_011982866.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H96_RS14485	54628..54873	-	246	WP_011982865.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6H96_RS14490	55034..55522	-	489	WP_049768461.1	helix-turn-helix transcriptional regulator	-
I6H96_RS14495	55701..56078	-	378	WP_011982863.1	hypothetical protein	-
I6H96_RS14500	56081..56251	-	171	WP_162024451.1	ribbon-helix-helix protein, CopG family	-
I6H96_RS14505	56499..57212	-	714	WP_011982861.1	ParA family protein	-
I6H96_RS14510	57691..58173	+	483	WP_011982860.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..170351	170351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10998.80 Da
• Isoelectric Point: 10.6834

>T183835 WP_011982866.1 NZ_CP066051:c54638-54351 [Brucella anthropi]
MPYKLEFLPSALKEWNKLGATIQQQLKKKLRERLETPRVVSASLHGMPDHYKIKLRQLGYRLVYSVNDDTVTVLVVAVGK
RERGDVYNTARSRTQ

Nucleotide

• Length: 288 bp

>T183835 NZ_CP087189:c758254-757952 [Pseudomonas protegens]
ATGTTCGTTTTCGAGCAGACTCCTGAGTTCAGCCGCTGGCTGCTGAACCTGAAGGACCCGCAAGGGAAAACCCGTGTACT
GGCCAGGATCCGCGCCGCACAGCTGGGCCGTTTTGGCGACTGCGAACCTGTGGGCGAAGGCGTCCACGAGATACGCATCC
ACCGCGGCCCGGGTTACCTGGTGTACTTCTCGCACCGCGGCAAAGTGATCTACCTGCTGTTGATCGGCGGCGACAAGGCC
ACCCAGAACCGCGATATCCAACGGGCCAGGCAACTGGCCTGCGACCTCAAGAGCGAGGGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8886.16 Da
• Isoelectric Point: 4.2813

>AT183835 WP_011982865.1 NZ_CP066051:c54873-54628 [Brucella anthropi]
MQRVEANIAVSVSDLKKSPSAVMDEANGEAVAVLNHNRIMAYMVPAGVYEAMLEQLDDIRLTEIIRNRADEKGISVDIDA
L

Nucleotide

• Length: 246 bp

>AT183835 NZ_CP087189:c757948-757658 [Pseudomonas protegens]
ATGCGCCCCCCAGTGACCCGCTTCGACGCGGCGGAGTACCTCAAGACCCCTGAAGACATGGTCGCCTACCTCGACGCCTG
CTTCGAGGAAGACACCGGCGACGGCGTGCTGATCCGCGCCGCACTCAACGACATCGCCCGTGCCCGCGGCATGACCCAGG
TCGCCCGTGACACCGGCCTGGGCCGCGAAAGCCTGTACAAGGCCCTCGGCAGCCAGGGCAACCCGGAGTTCGCGACTATC
ATCAAGGTGATGAAGGCCCTGGGGTTGAAGATCAACGTCAGCAACGCCTGA

Structures

Toxin

• AlphaFold DB: A6X7M9

Antitoxin

• AlphaFold DB: A6X7N0