Detailed information of TA system TA183833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1895720..1896417
• Replicon: chromosome
• Accession: NZ_CP066050
• Organism: Brucella anthropi strain FDAARGOS_1039

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6H96_RS09345
• Protein ID: WP_040130219.1
• Coordinates: 1895720..1896145 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A256H0E6
• Locus tag: I6H96_RS09350
• Protein ID: WP_012090891.1
• Coordinates: 1896145..1896417 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H96_RS09320	1890941..1891810	+	870	WP_049768389.1	carbohydrate ABC transporter permease	-
I6H96_RS09325	1891900..1893129	+	1230	WP_115179296.1	ABC transporter substrate-binding protein	-
I6H96_RS09330	1893323..1894630	-	1308	WP_012090894.1	IS1380 family transposase	-
I6H96_RS09335	1894971..1895252	+	282	WP_041544966.1	hypothetical protein	-
I6H96_RS09340	1895401..1895709	+	309	WP_012090893.1	HigA family addiction module antidote protein	-
I6H96_RS09345	1895720..1896145	-	426	WP_040130219.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6H96_RS09350	1896145..1896417	-	273	WP_012090891.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
I6H96_RS09355	1896471..1896758	-	288	WP_115179265.1	hypothetical protein	-
I6H96_RS09360	1897117..1898052	-	936	WP_012090889.1	isoaspartyl peptidase/L-asparaginase	-
I6H96_RS09365	1898095..1898913	-	819	WP_012090888.1	M55 family metallopeptidase	-
I6H96_RS09370	1898926..1899810	-	885	WP_012090887.1	ABC transporter permease subunit	-
I6H96_RS09375	1899807..1900727	-	921	WP_012090886.1	glutathione ABC transporter permease GsiC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1838728..1914273	75545
	-	flank	IS/Tn	-	-	1893323..1894630	1307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (1-79)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 14970.04 Da
• Isoelectric Point: 4.4540

>T183833 WP_040130219.1 NZ_CP066050:c1896145-1895720 [Brucella anthropi]
MIVDASAIIAILFDEVEAPECMDALQSDTARLISAVNYVEAGTVLAGRIREGDRHAAIADLDAFLADFGILVASIDDRLA
RAAMKARVEYGKGFGTRAGLNFGDCFAYALAKRHSAPLLYVGDDFALTDIQSALSRGPCQG

Nucleotide

• Length: 426 bp

>T183833 NZ_CP087189:180314-180595 [Pseudomonas protegens]
ATGACTTATAGCCTTGAGTTCCTGCCTTCGGCACACAAGGAATGGAACAAGTTGGGGCATACGCTTCGAGAGCAATTCAA
GAAAAAGCTCGCCGAACGGCTTGAGGTGCCGCGTATCCCTGCTGATGCCCTGCATGGGCTTCCCGATTGCCACAAAATTA
AACTCAAGGCTTCGTGCTACCGATTGGTTTATCAAGTGATCGAAGAGCGCGTCGTGGTTTGCGTGGTTGCGGTCGGCAAG
CGCGAGCGTGGAACCGTTTACGAGCAGGCCAGGAAACGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10588.79 Da
• Isoelectric Point: 5.9303

>AT183833 WP_012090891.1 NZ_CP066050:c1896417-1896145 [Brucella anthropi]
MALSIKTDEADRLARELSRLTGETMTDAITQAMRERLDRLRAEREAQRDYVARMKAFVRERASRYDRRPVTKQEWDEAVG
DTSEEPGYHQ

Nucleotide

• Length: 273 bp

>AT183833 NZ_CP087189:180079-180324 [Pseudomonas protegens]
ATGCAGAGTATTCTGGCTGACAAAGCCGTCAGCGTATCTGAGTTGAAGAAAAACCCTTCTGCCGTCTTGAGTGGTGCCCA
GGGCGGGCCAGTGGCGGTGTTGAATCACAATCGTGTCATGGGCTACATGGTTCCGGCCGAGGTTTTCGAAGCGTTGATGG
AACGCCTGGATGATCTGGAGTTGGCCGAAATCATCCGCGAGCGCAGCCATGAGACGCCCGTTCCGGTCAGTCTGGATGAC
TTATAG

Structures

Antitoxin

• AlphaFold DB: A0A256H0E6