Detailed information of TA system TA183832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 542617..543169
• Replicon: chromosome
• Accession: NZ_CP066050
• Organism: Brucella anthropi strain FDAARGOS_1039

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A6WZ45
• Locus tag: I6H96_RS02640
• Protein ID: WP_012091587.1
• Coordinates: 542909..543169 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A6WZ44
• Locus tag: I6H96_RS02635
• Protein ID: WP_012091586.1
• Coordinates: 542617..542922 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H96_RS02580	538239..538652	+	414	WP_012091579.1	hypothetical protein	-
I6H96_RS02585	538642..539136	+	495	WP_012091580.1	hypothetical protein	-
I6H96_RS02590	539133..539393	+	261	WP_012091581.1	hypothetical protein	-
I6H96_RS02595	539386..539589	+	204	WP_041545065.1	hypothetical protein	-
I6H96_RS02600	539586..539825	+	240	WP_012091582.1	hypothetical protein	-
I6H96_RS02605	539837..540295	+	459	WP_012091583.1	deoxycytidine triphosphate deaminase	-
I6H96_RS02610	540681..540926	-	246	WP_049768397.1	DUF982 domain-containing protein	-
I6H96_RS02615	541005..541220	-	216	WP_041545071.1	hypothetical protein	-
I6H96_RS02620	541264..541941	-	678	WP_012091585.1	SOS response-associated peptidase	-
I6H96_RS02625	541948..542181	-	234	WP_041545073.1	hypothetical protein	-
I6H96_RS02630	542183..542410	-	228	WP_041545074.1	hypothetical protein	-
I6H96_RS02635	542617..542922	-	306	WP_012091586.1	BrnA antitoxin family protein	Antitoxin
I6H96_RS02640	542909..543169	-	261	WP_012091587.1	BrnT family toxin	Toxin
I6H96_RS02645	543272..543790	-	519	WP_012091588.1	hypothetical protein	-
I6H96_RS02650	543819..546089	-	2271	WP_012091589.1	AAA family ATPase	-
I6H96_RS02655	546091..546402	-	312	WP_012091590.1	hypothetical protein	-
I6H96_RS02660	546416..546871	-	456	WP_012091591.1	single-stranded DNA-binding protein	-
I6H96_RS02665	546883..547149	-	267	WP_012091592.1	hypothetical protein	-
I6H96_RS02670	547142..547348	+	207	WP_125334435.1	hypothetical protein	-
I6H96_RS02675	547470..548021	-	552	WP_041545080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	501216..564849	63633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (29-93)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9942.49 Da
• Isoelectric Point: 8.5409

>T183832 WP_012091587.1 NZ_CP066050:c543169-542909 [Brucella anthropi]
MKIIWDETKRLTNLEKHKGFDFADLTTEFFDNAAIYPAKQGRLMAVGEFRGETIIATIFVLLGTEALSVISMRRASRKER
KLYDEQ

Nucleotide

• Length: 261 bp

>T183832 NZ_CP087188:c4674309-4674040 [Pseudomonas laurylsulfatiphila]
ATGCTGGTTGAGTGGCGGCCCGAAGCACGGGCCGAACTCTGGCAAATCCTGAATTACATTGGCGACCGAAACTTCGTAGC
GGCCAGCGAGCTATTTCAAGCCATTGAGGCTGCTACATCGTCCCTTCCCGAACATCCCTACCTTTATCGATTCGGTCGAG
TACTCGGCACTCGGGAGATTGTTGTGCATCCCAACTACCTGGTGGTTTACCGAGTGACGGATCGAATCGAGATATTGAGC
GTGTTGCATGCTCGACAGGAATATCCCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11220.70 Da
• Isoelectric Point: 10.2394

>AT183832 WP_012091586.1 NZ_CP066050:c542922-542617 [Brucella anthropi]
MTNNKRKAIRPLTDAEEAEIQREIAADPDAPELTDEQATQRMTFAEAVRRTRGPGKKAPKETVTLRLDPDVVEAFKADGP
GWQGRVNEALRKAKKLPNRAA

Nucleotide

• Length: 306 bp

>AT183832 NZ_CP087188:c4674490-4674299 [Pseudomonas laurylsulfatiphila]
ATGAGCACCGAACTTTCCCCCATCGTTTCAGAGTTTGAGACCAAAGAGCAGGCCGCCAGCTATGACCGTTGGTTCCGCGC
CAAGGTCCAGGCTTCGCTTGACGACCCACGCCCGAATGTCCCGCACGATCAGGTGATGGCCGATATGCGAGCCCTCATCG
AATCAAAGCGCAACAAGTACGATGCTGGTTGA

Structures

Toxin

• AlphaFold DB: A6WZ45

Antitoxin

• AlphaFold DB: A6WZ44