Detailed information of TA system TA183822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1529942..1530627
• Replicon: chromosome
• Accession: NZ_CP066037
• Organism: Burkholderia ambifaria strain FDAARGOS_1027

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q0BFH0
• Locus tag: I6H84_RS07165
• Protein ID: WP_011656832.1
• Coordinates: 1529942..1530364 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q0BFH1
• Locus tag: I6H84_RS07170
• Protein ID: WP_011656831.1
• Coordinates: 1530361..1530627 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H84_RS07145	1526044..1527114	+	1071	WP_011656836.1	hypothetical protein	-
I6H84_RS07150	1527083..1527988	+	906	WP_011656835.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
I6H84_RS07155	1528276..1529328	-	1053	WP_011656834.1	transposase	-
I6H84_RS07160	1529421..1529861	+	441	WP_011656833.1	MaoC family dehydratase	-
I6H84_RS07165	1529942..1530364	-	423	WP_011656832.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6H84_RS07170	1530361..1530627	-	267	WP_011656831.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6H84_RS07175	1530905..1531921	-	1017	WP_011656830.1	acetyltransferase	-
I6H84_RS07180	1531921..1532760	-	840	WP_006755049.1	folate-dependent phosphoribosylglycinamide formyltransferase PurN-like protein	-
I6H84_RS07185	1532847..1535123	-	2277	WP_175783450.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14774.86 Da
• Isoelectric Point: 7.8895

>T183822 WP_011656832.1 NZ_CP066037:c1530364-1529942 [Burkholderia ambifaria]
VTTLYMLDTNICSFIMRERPSAVLSRLQSCVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVSRLDGVLSWDTA
AVDATTAIRVDLAARGTPIGANDASIAGHAIAAGAVLVTNNSREFSRVAGLSLEDWAASA

Nucleotide

• Length: 423 bp

>T183822 NZ_CP087187:2167226-2167501 [Pseudomonas atacamensis]
ATGGAGTTGAAGTGGACCAGCAAAGGGCTTTCCGACCTGACCAGGCTTTATGAGTTTTTGGCAGCAGTTAATCAACCTGC
CGCTGCGCGCACTGTGCAACAGCTGACCGCCGCTCCGATAATGCTGCTAGCCAATCCGCGCTTGGGTGAAAGTCTGGAAG
AATTTGAACCGCGAGATGTTCGAAGGATTCTGGTCGGGCACTACGAAATGCGCTACGAAATTGTCGATTCGACTATCTAC
CTGCTGCGCCTTTGGCACACTCGCGAAGAACGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10017.29 Da
• Isoelectric Point: 4.5498

>AT183822 WP_011656831.1 NZ_CP066037:c1530627-1530361 [Burkholderia ambifaria]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLLLRPVRPTWLSFATEPRADADFLVERPTVVEAGRFELPGADD
DAQTESAR

Nucleotide

• Length: 267 bp

>AT183822 NZ_CP087187:2166952-2167224 [Pseudomonas atacamensis]
ATGGCCGCGCCAATGAAAACCCGATCCGTGACTGCCCATGTACCCGTGCAATTGGCCGAAAAGGTCGATCTGATGGCTGA
GCGTCTGGAACGCTCGCGTAACTGGATTGTGAAACAGGCCCTGAGCGCCTGGATTGATCAAGAGGAAGAGCGCAGCCGTT
TGACGCGCGAAGCCTTGGCTGATGTGGACGCCGGGCGCGTTATCGATCATCAATCAGTGCAGGCATGGGCGGACAGCTTG
AGTTCCGATACGCCTCTCAAGGCTCCACTTTGA

Structures

Toxin

• AlphaFold DB: Q0BFH0

Antitoxin

• AlphaFold DB: Q0BFH1