Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 5118057..5118675 Replicon chromosome
Accession NZ_CP066032
Organism Escherichia coli strain FDAARGOS_1059

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6I16_RS25390 Protein ID WP_001291435.1
Coordinates 5118457..5118675 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6I16_RS25385 Protein ID WP_000344800.1
Coordinates 5118057..5118431 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I16_RS25375 5113146..5114339 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6I16_RS25380 5114362..5117511 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I6I16_RS25385 5118057..5118431 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6I16_RS25390 5118457..5118675 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6I16_RS25395 5118847..5119398 + 552 WP_000102564.1 maltose O-acetyltransferase -
I6I16_RS25400 5119514..5119984 + 471 WP_000136192.1 YlaC family protein -
I6I16_RS25405 5120148..5121698 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6I16_RS25410 5121740..5122093 - 354 WP_000878141.1 DUF1428 family protein -
I6I16_RS25420 5122472..5122783 + 312 WP_000409911.1 MGMT family protein -
I6I16_RS25425 5122814..5123386 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T183814 WP_001291435.1 NZ_CP066032:5118457-5118675 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T183814 NZ_CP087186:c4813714-4813370 [Pseudomonas siliginis]
ATGCGAACAATCTTTTTTGAAACAACGATATTTACCAAAAGCGTTGGCCGCTATCTGACGGACGACGAATATCGAACATT
GCAGAACTACCTTCAGAGCAATCCGCTTGCGGGCGATGTAATGCCTCGAACGGGTGGCTTCCGTAAGCTGCGCTGGGCTG
ATTCTCGCCGTGGCAAGGGAAGACGAGGCGGCTTGCGGGTTCTCTACTATTGGCTTATGAATGATGGTCAGTTCTGGCTG
TTCGCCATTTACGACAAAGATGAGCTTGAGAAGCTGACTGCCGAGCAAGAGAGGGCACTCAGGACAGCCATTGATAAAGA
GTTGAAAAAACGAGGTACCCCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT183814 WP_000344800.1 NZ_CP066032:5118057-5118431 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT183814 NZ_CP087186:c4813373-4813059 [Pseudomonas siliginis]
ATGAAAAAACGCGACCTGTTTGCAGAGTTAATGCAGGGCGTTGATGAGATGGCGGCTCAACGCGAGGGCAAGATCACACT
GCGCAATACAGTGCTTGAGGACAAGCCTGTACCTGAAGTGGGCGCACAGGAAATTGTGGCTCTTCGAGAAAAACTGCATA
TGTCTCAAGCTGTTTTTGCCAGTCGAATCAGGACAAGTCCAAACACCCTTCGAAATTGGGAGCAGGAAAAGTCGAAGCCC
AATGCTCAAGCAGCATTATTGATACGACTGGTTGAAAAATTTCCAGATATGGTTGAGCGCTTGAACACTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References