Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2193336..2193990 Replicon chromosome
Accession NZ_CP066032
Organism Escherichia coli strain FDAARGOS_1059

Toxin (Protein)


Gene name cptA Uniprot ID A0A0K3MKC4
Locus tag I6I16_RS10575 Protein ID WP_000244768.1
Coordinates 2193583..2193990 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6I16_RS10570 Protein ID WP_000354046.1
Coordinates 2193336..2193602 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I16_RS10550 2189623..2189934 + 312 WP_001182946.1 N(4)-acetylcytidine aminohydrolase -
I6I16_RS10555 2190098..2190757 + 660 WP_000250274.1 hemolysin III family protein -
I6I16_RS10560 2190880..2191860 - 981 WP_112081489.1 IS110-like element IS621 family transposase -
I6I16_RS10565 2192113..2193093 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I6I16_RS10570 2193336..2193602 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6I16_RS10575 2193583..2193990 + 408 WP_000244768.1 protein YgfX Toxin
I6I16_RS10580 2194030..2194551 - 522 WP_001055874.1 flavodoxin FldB -
I6I16_RS10585 2194663..2195559 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6I16_RS10590 2195584..2196294 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6I16_RS10595 2196300..2198033 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2190880..2191860 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15994.97 Da        Isoelectric Point: 10.5673

>T183796 WP_000244768.1 NZ_CP066032:2193583-2193990 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINACQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T183796 NZ_CP087183:c3928149-3927712 [Pseudomonas putida]
GTGATTTTGTGGCGTATCAGCGCCTATGCAGATTTGAGTGGCACAGGGGGGTTGCGTGTAAGCGGCCGCTGGCACCAGGC
GGGCCGGCCCGTGGTGTATGCCGCCACCAGCCCTTCCGGGGCCATGCTCGAGGTGCTGGTGCACCTGGAGATCGACCCGG
AGGATTTTCCTACCACCATGCGCCTGATCCGCATCGAATTGCCCGACACGGTTTCACAAGCGCAGCTGCCTGCCTTGCAG
CCCGGCTGGTCCGCTCAGCCTGAGTTGACCCGGGCGCTGGGCAATCGCTTTCTGGATGACTGCTCGGCTTTGCTGCTGCC
CGTGCCGAGCGCGATCATGCCCAGTACCATCAACTACCTGTTCAACCCACGGCACGCACAGGCGCAGCGCGCGAAAATCC
AGGTCGAGGATTTTACCCCGGACAGCCGCTTGTTCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT183796 WP_000354046.1 NZ_CP066032:2193336-2193602 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT183796 NZ_CP087183:c3928595-3928146 [Pseudomonas putida]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGGAGGCGCTGCTGGAAATACCCGAACT
TGCCAGTGACTTCGAAATCCACACCCGCATCACCCATGGTTTTGCTGCCACCTGGCTGGTGAAGCTGACCGAGCGTGGTG
TGTTCACGCCGGTGGAGCGGGACCAGATCATTCCCCTGCGCACTCTGAAAACCCGTATCGAGCGTGACCAGCCGCTGACC
GTGGAAGAGAGTGATCGGCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAAGCCGTGTTCGGTGAGGCGGACAAGGC
CAAGCGCTGGCTGTCCAAGCCCAAGGAACGCTTCTCGGGGCTGACGCCCATGCAGATGCTCACCACCCAGCAAGGCACCA
CTCAGGTCGAAGAGATGCTGCTGCAGATTGCCGAGGGCTTTGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K3MKC4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References