Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 740797..741294 Replicon chromosome
Accession NZ_CP066032
Organism Escherichia coli strain FDAARGOS_1059

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6I16_RS03505 Protein ID WP_001173343.1
Coordinates 740797..740970 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6I16_RS03510 Protein ID WP_000398619.1
Coordinates 740998..741294 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I16_RS03495 737467..738924 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
I6I16_RS03500 739161..740678 + 1518 WP_001295074.1 lysine--tRNA ligase -
I6I16_RS03505 740797..740970 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6I16_RS03510 740998..741294 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6I16_RS03515 741521..741793 - 273 WP_000405647.1 N-acetyltransferase -
I6I16_RS03520 741805..742035 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
I6I16_RS03525 742216..743847 + 1632 WP_001216487.1 sensor histidine kinase -
I6I16_RS03530 743844..744563 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 724818..741294 16476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T183791 WP_001173343.1 NZ_CP066032:c740970-740797 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T183791 NZ_CP087182:4264687-4264866 [Pseudomonas protegens]
ATGAAATTCAGCGAATTCAGACGGTGGTTGAAAGCTCAAGGCGTGGTCTTCGAGGCCGGTAAAGGCAGCCACTTCAAAGT
CACGGCCCCAAACGGCAACAAGACCACCTTCGCGGATCACGGCAGCAAGGAAATGCCCGAACCCACCCGCAAGGCAATCA
TCAGGCAATTGGGGCTCTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT183791 WP_000398619.1 NZ_CP066032:c741294-740998 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT183791 NZ_CP087182:4264917-4265342 [Pseudomonas protegens]
ATGTACGACTACGCCATTCGCTTCGAGCAAGACCGTGCACCGGGAGTGGCGGTCTTCTGCCGCGATCTGCCCGAACTCAA
TAGCTTTGGCGATGATCCTGAGCACGCCATCAACCAGGCCATCGACGCGCTCGAAACCACGCTGTCCCTTTACGTCGACC
AGCGCCGCGCCATTCCCCCAGCCTCTGAGCCGCTACCAGGGGAACAGGTCATTCATTTGCCAATGGTGACCGTGGCCAAG
ATAGCGCTGTGGAACACGATGATGGAGCGGGACATGCGCAAGGCCGATCTATGCCGACTGCTCGGAATCCACCAGGCCCA
GGGAGATCGCCTGGTGGACTTTCTACACAGCTCCAAAATGGAACAGCTGGAAAATGCCCTCGCAGCCCTAGGTAAACGTC
TTGCAGTATCAGTGGAGGCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References