Detailed information of TA system TA183789

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 482089..482921
• Replicon: chromosome
• Accession: NZ_CP066032
• Organism: Escherichia coli strain FDAARGOS_1059

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: I6I16_RS02205
• Protein ID: WP_000854765.1
• Coordinates: 482089..482463 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: I6I16_RS02210
• Protein ID: WP_001295723.1
• Coordinates: 482553..482921 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I16_RS02185	477546..480662	+	3117	WP_000148752.1	HsdR family type I site-specific deoxyribonuclease	-
I6I16_RS02190	481150..481308	-	159	WP_001467148.1	hypothetical protein	-
I6I16_RS02195	481408..481584	-	177	WP_000839290.1	DUF957 domain-containing protein	-
I6I16_RS02200	481601..482092	-	492	WP_000976842.1	hypothetical protein	-
I6I16_RS02205	482089..482463	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
I6I16_RS02210	482553..482921	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6I16_RS02215	483084..483305	-	222	WP_000692345.1	DUF987 domain-containing protein	-
I6I16_RS02220	483368..483844	-	477	WP_001186777.1	RadC family protein	-
I6I16_RS02225	483860..484333	-	474	WP_000855059.1	antirestriction protein	-
I6I16_RS02230	484675..485493	-	819	WP_001234719.1	DUF945 domain-containing protein	-
I6I16_RS02235	485648..485806	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T183789 WP_000854765.1 NZ_CP066032:c482463-482089 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T183789 NZ_CP087182:c2508371-2508189 [Pseudomonas protegens]
GTGCAAAGCAGACTGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGAGTCACCGGCAGCCATCATCTGTT
CCAGCATCCCTACCGGCCCAACACCGTTCCCGTGCCGCATCCGAAGAAGGATCTGCCACGGGGAACGGTCCGGGCCATCA
GGAAACTGGCCGGGCTGCTCTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT183789 WP_001295723.1 NZ_CP066032:c482921-482553 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT183789 NZ_CP087182:c2508150-2507749 [Pseudomonas protegens]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACGGCCACCGGCATCCAGATTCCCGACATTCCCGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCCTACAGCGCCGCCGTCGAGATTGCCCACCTCATGCTCGAAGAGCTGGCCCGCG
CCGGGCAGGCCATTCCCCTGCCCTCTGCCACCCAAACGCACCGCGCCCATCCTGATTACCAGGGGATGGGCTGGGGCATG
CTGGAGATCGACATCACGCCTTACCTGGGCAAGACCGAGAAGGTCAACGTGACCTTGCCGGGGTACGTGATTCAGCAGAT
CGACCGCTTTGTCCGAGCGCACAACATCAAGAGCCGGTCGTCCTTTCTGGCGGATGCGGCGCTGGAAAAGCTCGGGCGCT
AA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52