Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 434992..435568 Replicon chromosome
Accession NZ_CP066032
Organism Escherichia coli strain FDAARGOS_1059

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag I6I16_RS01975 Protein ID WP_001297643.1
Coordinates 435281..435568 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag I6I16_RS01970 Protein ID WP_000063156.1
Coordinates 434992..435294 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I16_RS01955 431588..433738 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
I6I16_RS01960 433788..433991 + 204 WP_000467859.1 YbdD/YjiX family protein -
I6I16_RS01965 434002..434958 + 957 WP_001297640.1 GTPase -
I6I16_RS01970 434992..435294 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
I6I16_RS01975 435281..435568 - 288 WP_001297643.1 BrnT family toxin Toxin
I6I16_RS01980 435767..436464 - 698 Protein_384 IS1 family transposase -
I6I16_RS01985 436521..436616 + 96 Protein_385 dienelactone hydrolase family protein -
I6I16_RS01990 436762..437994 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
I6I16_RS01995 438035..439315 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T183788 WP_001297643.1 NZ_CP066032:c435568-435281 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T183788 NZ_CP087182:1017462-1017740 [Pseudomonas protegens]
ATGAGACAGCTGAAATGGACGAGCAAGGCCCTATCCGATCTGGCTCGGCTTTTTGAGTTTCTCGCCCAGGCCAATCGTTC
TACTGCTGCTCGGACCGTGCAATCGCTGGTGCAGGCTCCAGATGCTCTTCTATCTAACCCACGATTGGGAGAGCAGTTGG
AGGAATTCAAGCCGAGGGATGTTCGACGATTGCTGGTGGGGCATTACGAGATGCGTTATGAGATTCAAGGCATGGCCTTG
TCCATCCTGCGTATTTGGCATGTCCGGGAAGATCGTTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT183788 WP_000063156.1 NZ_CP066032:c435294-434992 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT183788 NZ_CP087182:1017202-1017465 [Pseudomonas protegens]
ATGCCCGCGACAAAAGTACTGACCGCTCACATTCCCTTGGACCTGGCTGACAAGGTCGATCAACTTGCTTCTCGCCTGGA
ACGCTCCCGGGCCTGGGTCGTGAAGCAGGCGTTGGTGGCCTGGGTTGACCAGGAAGAAGAGCGCCGTCGACTGACGCTTG
AGGCCTTGGCAGATGTCGATGCTGGAAGGGTTGTGGATCATCAGTCAGTGTTGGCTTGGGCGCAGAGTCTGTCTGGCGAC
AACCCTCTGCCCTTACCAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References