Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2612293..2612936 Replicon chromosome
Accession NZ_CP066019
Organism Cupriavidus necator strain FDAARGOS_1030

Toxin (Protein)


Gene name hicA Uniprot ID A0A7T4KLX9
Locus tag I6H87_RS30720 Protein ID WP_041688562.1
Coordinates 2612293..2612469 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R7XL07
Locus tag I6H87_RS30725 Protein ID WP_010810694.1
Coordinates 2612520..2612936 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H87_RS30700 2607927..2608745 + 819 WP_011617819.1 flagellar assembly protein FliH -
I6H87_RS30705 2608738..2610213 + 1476 WP_010810690.1 flagellar protein export ATPase FliI -
I6H87_RS30710 2610191..2610649 + 459 WP_011617820.1 flagellar export protein FliJ -
I6H87_RS30715 2610675..2612093 + 1419 WP_011617821.1 flagellar hook-length control protein FliK -
I6H87_RS30720 2612293..2612469 + 177 WP_041688562.1 type II toxin-antitoxin system HicA family toxin Toxin
I6H87_RS30725 2612520..2612936 + 417 WP_010810694.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I6H87_RS30730 2612967..2613629 + 663 WP_011617823.1 DNA oxidative demethylase AlkB -
I6H87_RS30735 2613714..2614202 + 489 WP_041688226.1 GFA family protein -
I6H87_RS30740 2614252..2615169 - 918 WP_010810697.1 cysteine synthase A -
I6H87_RS30745 2615502..2616542 + 1041 WP_011617825.1 site-specific integrase -
I6H87_RS30750 2616867..2617601 + 735 WP_041688227.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(87-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6638.70 Da        Isoelectric Point: 12.0931

>T183780 WP_041688562.1 NZ_CP066019:2612293-2612469 [Cupriavidus necator]
VKRSEFRRWLAGQGATFSEGAKHVRVYLNGRKTTLPRHPSHEIREGLRKAILKQLGLS

Download         Length: 177 bp

>T183780 NZ_CP066019:2612293-2612469 [Cupriavidus necator]
GTGAAGCGGAGTGAATTCCGGCGATGGCTCGCCGGACAGGGCGCCACCTTCAGTGAAGGCGCGAAGCATGTGAGGGTGTA
TCTCAATGGCAGGAAGACCACGCTGCCACGGCATCCCAGCCACGAGATCCGGGAGGGGTTGCGCAAGGCGATTCTCAAGC
AACTCGGCCTGTCATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15083.34 Da        Isoelectric Point: 5.2420

>AT183780 WP_010810694.1 NZ_CP066019:2612520-2612936 [Cupriavidus necator]
MLRFPANIVPDDGGFAVSFPDIPEALTSGDTIEQAREMAADALATAMEFYFEDGRPVPLPSKAKRGQHLVELPASVSAKV
LLLNEMIAQGVTQAELARRLNTRKQEVQRIVNLDHATKIDTIEAAFRALGKRLELTVA

Download         Length: 417 bp

>AT183780 NZ_CP066019:2612520-2612936 [Cupriavidus necator]
ATGCTGCGTTTCCCTGCCAACATCGTCCCGGACGACGGTGGCTTTGCTGTGTCGTTTCCCGATATCCCCGAAGCCCTGAC
CAGCGGCGACACCATCGAGCAGGCCCGCGAAATGGCCGCCGATGCGCTCGCCACGGCCATGGAATTCTATTTCGAAGATG
GCCGCCCGGTGCCACTGCCGTCGAAGGCAAAACGCGGCCAGCATCTGGTTGAACTGCCGGCCAGCGTGTCGGCGAAGGTG
CTCCTGCTTAACGAGATGATCGCGCAGGGCGTGACGCAGGCGGAACTGGCGCGCCGGCTGAATACGCGCAAGCAAGAGGT
GCAACGTATCGTCAACCTGGACCATGCGACCAAGATCGATACGATCGAGGCTGCATTCCGGGCGCTGGGGAAGCGGCTGG
AACTGACGGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T4KLX9

Antitoxin

Source ID Structure
AlphaFold DB A0A7T4KMX9

References