Detailed information of TA system TA183775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4002917..4003510
• Replicon: chromosome
• Accession: NZ_CP066018
• Organism: Cupriavidus necator strain FDAARGOS_1030

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q0KBP4
• Locus tag: I6H87_RS18820
• Protein ID: WP_011615086.1
• Coordinates: 4002917..4003216 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: I6H87_RS18825
• Protein ID: WP_010810129.1
• Coordinates: 4003220..4003510 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H87_RS18800	3998287..3998913	-	627	WP_010810124.1	hypothetical protein	-
I6H87_RS18805	3998922..4000106	-	1185	WP_011615089.1	acetyl-CoA C-acyltransferase family protein	-
I6H87_RS18810	4000198..4001577	-	1380	WP_041687282.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6H87_RS18815	4001712..4002680	-	969	WP_011615087.1	tRNA-dihydrouridine synthase	-
I6H87_RS18820	4002917..4003216	+	300	WP_011615086.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H87_RS18825	4003220..4003510	+	291	WP_010810129.1	putative addiction module antidote protein	Antitoxin
I6H87_RS18830	4003567..4004118	-	552	WP_010810130.1	polyhydroxyalkanoate synthesis repressor PhaR	-
I6H87_RS18835	4004555..4005295	-	741	WP_010810131.1	3-ketoacyl-ACP reductase	-
I6H87_RS18840	4005370..4006551	-	1182	WP_010810132.1	acetyl-CoA C-acetyltransferase	-
I6H87_RS18845	4006636..4008405	-	1770	WP_011615085.1	class I poly(R)-hydroxyalkanoic acid synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11056.61 Da
• Isoelectric Point: 10.1574

>T183775 WP_011615086.1 NZ_CP066018:4002917-4003216 [Cupriavidus necator]
MLSIRTTQISDRWYSALRDHVARIRIQVRVDRLLNGNPGDAKALGGGVSEMRVDHGPGYRIYFQQRGQVIVILLCGGNKS
TQHADIERARSLAANLDLE

Nucleotide

• Length: 300 bp

>T183775 NZ_CP087178:1018054-1018347 [Pseudomonas sp. B21-036]
GTGATCTATTCCATAGAACAGACTTTGGCATTCAGCGGCTGGCTTCTGGCGCTACGCGATGTACGAGCGAGGATCGCCAT
TGGTCGACGCATCGAACGTGCGCTTGCGGGCAACTTGGGGGATACGAAGGCGGTTGGTGATGGTGTTTCAGAGATGCGCA
TCGATTCCGGCCCAGGCTATCGGGTGTACTTCACCGTGCGTAGCGGCGTAGCAATCTTGTTGCTGACAGGCGGGGACAAG
TCTTCTCAAGCAGCAGATATCCGGATGGCCAAGCGGTTGGCCAAAGAGGTTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10607.97 Da
• Isoelectric Point: 6.4635

>AT183775 WP_010810129.1 NZ_CP066018:4003220-4003510 [Cupriavidus necator]
MKEPTRPWDSAEHLRNDQEIAAYLDACLAEAGDDDRFIAYALGVVARARGMTRLARETGLSRESLYRSLSGEGNPEFGTI
WKVMRALGIRLHASAG

Nucleotide

• Length: 291 bp

>AT183775 NZ_CP087178:1018350-1018649 [Pseudomonas sp. B21-036]
ATGAGCGTTACATTGATTCCATTCGACATGGCGGCACTGCTTGATAGTGATGAAGCCATCAGCGAGTACTTGTCTCAGGT
GCTGGCAGAGGGCGACAGCGACGAGCTTTTACGTGCAATAGGGTACGTGGCCAAAGCGCGCGGAATGTCGCTGGTTGCCA
AAGAGACTGGATTGGGAAGAGAGAGCCTTTACAAGGCCTTCGCGCCAGGCGCCAAGCCTCGTTTCGATACGGTGCTGAAG
GTGATGCATGCGCTGGGAATCGAACTCTGTGCGCGCTCGCATCGAGGGGCTTCGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A7T4KIZ7