Detailed information of TA system TA183770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2606044..2606681
• Replicon: chromosome
• Accession: NZ_CP066015
• Organism: Kytococcus sedentarius strain FDAARGOS_1061

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C7NJK8
• Locus tag: I6I18_RS12460
• Protein ID: WP_012801756.1
• Coordinates: 2606289..2606681 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: I6I18_RS12455
• Protein ID: WP_041290823.1
• Coordinates: 2606044..2606292 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I18_RS12435	2601807..2603051	-	1245	WP_012801751.1	cupin-like domain-containing protein	-
I6I18_RS12440	2603048..2603362	-	315	WP_169307763.1	hypothetical protein	-
I6I18_RS12445	2603813..2605135	+	1323	WP_049758591.1	replicative DNA helicase	-
I6I18_RS12450	2605170..2605982	-	813	WP_012801754.1	fused MFS/spermidine synthase	-
I6I18_RS12455	2606044..2606292	+	249	WP_041290823.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
I6I18_RS12460	2606289..2606681	+	393	WP_012801756.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6I18_RS12465	2606686..2607456	-	771	WP_012801757.1	DUF1524 domain-containing protein	-
I6I18_RS12470	2607575..2608234	-	660	WP_012801758.1	maleylpyruvate isomerase family mycothiol-dependent enzyme	-
I6I18_RS12475	2608284..2608625	-	342	WP_012801759.1	DUF2200 domain-containing protein	-
I6I18_RS12480	2608661..2609377	-	717	WP_081439726.1	HNH endonuclease	-
I6I18_RS12485	2609319..2609981	-	663	WP_012801761.1	FMN-binding protein	-
I6I18_RS12490	2610025..2610147	-	123	WP_115306804.1	Ltp family lipoprotein	-
I6I18_RS12495	2610242..2610637	+	396	Protein_2445	transposase family protein	-
I6I18_RS12500	2610671..2611081	-	411	Protein_2446	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Antitoxin VapB-like (1-81)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 13744.49 Da
• Isoelectric Point: 4.3094

>T183770 WP_012801756.1 NZ_CP066015:2606289-2606681 [Kytococcus sedentarius]
VIVDSSALVAILLAEPERQALLEAIQQAPEAAMTAASYLEVGMVVDGRGNPVLSRQYDALLEALGIEVVDTTAEQARVAR
AAHRDFGRGSGHPARLNFGDCLSYAAAVQDGVPLLFTGDDFIHTDVTPAR

Nucleotide

• Length: 393 bp

>T183770 NZ_CP087177:3439107-3439328 [Pseudomonas moraviensis]
ATCGAATACGTTGAACAACGCAACACTGTCGCTTCACTTGCACTGCACCAAAAGCTCAGCGCGGCAGCGCGAAGGCTTTC
CTGGATACCGCATGGTTTCCGCTCCGGCCGAATACCGGGCACTCGCGAAATGGTCATTAACTCGAATTATTTACTGGTCT
ATCGGGTGACAGACCAGATCAATATTCTGACCGTATTGCACGCCCGTAAAAAATATCCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9004.15 Da
• Isoelectric Point: 4.6158

>AT183770 WP_041290823.1 NZ_CP066015:2606044-2606292 [Kytococcus sedentarius]
MALTIKNDRVCDLAREAAQRSGQSQVSVIEQALERYLADHAEPVGRRVDEVLARIDASLAPGDAAAMRVELDRLYDERGL
PA

Nucleotide

• Length: 249 bp

>AT183770 NZ_CP087177:3438875-3439069 [Pseudomonas moraviensis]
ATGAGCGCTGAACTTTCACTTATCGTTTCAGACTTCGAAACCGAAGAAGAGGCTGCGAGCTATGACCGTTGGTATCGGGC
CAAGGTACAAGCCGCACTTGATGATCCACGCCCGCCTGTTCCCCACGACGAGGCAATGGTGCTGCTAGGCCAGATGCTGG
AAGAGAGGCGCAAGAATCGACGCGCTGCCGGTTAA

Structures

Toxin

• AlphaFold DB: C7NJK8