Detailed information of TA system TA183727

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4924092..4924786
• Replicon: chromosome
• Accession: NZ_CP065970
• Organism: Escherichia coli strain FDAARGOS_1058

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A2A3UTQ9
• Locus tag: I6I15_RS24190
• Protein ID: WP_001094399.1
• Coordinates: 4924092..4924460 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: I6I15_RS24195
• Protein ID: WP_077249034.1
• Coordinates: 4924481..4924786 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I15_RS24170	4919265..4920221	+	957	WP_000643336.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
I6I15_RS24175	4920218..4922416	+	2199	WP_000667031.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
I6I15_RS24180	4922426..4923382	+	957	WP_000121355.1	molybdenum cofactor insertion chaperone PaoD	-
I6I15_RS24185	4923433..4923771	+	339	Protein_4605	LysR family transcriptional regulator	-
I6I15_RS24190	4924092..4924460	-	369	WP_001094399.1	TA system toxin CbtA family protein	Toxin
I6I15_RS24195	4924481..4924786	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6I15_RS24200	4924890..4925534	-	645	WP_000086755.1	hypothetical protein	-
I6I15_RS24205	4925553..4925774	-	222	WP_000692315.1	DUF987 domain-containing protein	-
I6I15_RS24210	4925843..4926319	-	477	WP_001186742.1	RadC family protein	-
I6I15_RS24215	4926334..4926819	-	486	WP_000214318.1	antirestriction protein	-
I6I15_RS24220	4926910..4927728	-	819	WP_001234748.1	DUF945 domain-containing protein	-
I6I15_RS24225	4927819..4928052	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4898683..4934924	36241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13568.73 Da
• Isoelectric Point: 7.7465

>T183727 WP_001094399.1 NZ_CP065970:c4924460-4924092 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T183727 NZ_CP087169:283369-283722 [Pseudomonas sp. B21-047]
ATGCGAGTGGTAACAAAGACGGCGGTTGCACAAGCCATTGAAACGTACAGCCAGTGGAAAGAGCCACTGAACTTGTGGCT
GACCACCTTTGACAGGCCGCCCCTTCGGTTTGAGTCCTTTGATCAAATTCGTGATGTTTGGGGCACTGCCTCAGGATGGA
ACGTCGATCGCATTCCTCACTCAAAGCTGAGACACACGAGCAAAAAAGGACCTCTTGATATCTACGTCTTCGATATCAAG
AAAAATGAATGTCGAATCATCGCGTGGATCAACACACGAACCGGCACCATTTACATGAAGGACATTCTGCCCCACGCTGG
ATATGACAAGTGGTGCAAAAGTGATGTTAAGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT183727 WP_077249034.1 NZ_CP065970:c4924786-4924481 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT183727 NZ_CP087169:283774-284247 [Pseudomonas sp. B21-047]
ATGAAGCGTGATAAGATCGAAGCCAATGGACTAGATTCCTTCATAGCTAATATTTCACCAATGTTTGATGGGCTGAGTGA
TCAGATAGCTACAATGTCCAAGTTGTTATCTGCCTGTCATGATCCCATTGAAGATGATGATGACCTGCAGGGGCGGATGG
CGCTAATTGATGAGCTCTACGCTCATGCGGACAGTGAAGATCACGCAGCAGCACGTTTTGCGGAGCTGGTCGCTGATCGC
GTGTACGAGTACGAAACTGAAACGGTGTTGATACCTCAATCCTCTCAGTCGGAAGCGCTCGCGTTTCTGATTTCGGACAG
GGGCGTTAAACAAAAAGATTTGTCTGGCATTGCAACGCAAAGTGCTGTTTCTGAAATACTGAACAACAAAAGAAAAATGA
CGGTTGGGCAAATAAAAGGCTTTGCTGATTTCTTTAAGGTGCCTGTTGAATTCTTTATGAATGGTGCAGTCTGA

Structures

Toxin

• AlphaFold DB: A0A2A3UTQ9