183726

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4741999..4742617 Replicon chromosome
Accession NZ_CP065970
Organism Escherichia coli strain FDAARGOS_1058

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6I15_RS23335 Protein ID WP_001291435.1
Coordinates 4742399..4742617 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6I15_RS23330 Protein ID WP_000344800.1
Coordinates 4741999..4742373 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I15_RS23320 4737088..4738281 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6I15_RS23325 4738304..4741453 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I6I15_RS23330 4741999..4742373 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6I15_RS23335 4742399..4742617 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6I15_RS23340 4742789..4743340 + 552 WP_094250003.1 maltose O-acetyltransferase -
I6I15_RS23345 4743456..4743926 + 471 WP_000136192.1 YlaC family protein -
I6I15_RS23350 4744090..4745640 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6I15_RS23355 4745682..4746035 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6I15_RS23365 4746414..4746725 + 312 WP_000409911.1 MGMT family protein -
I6I15_RS23370 4746756..4747328 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T183726 WP_001291435.1 NZ_CP065970:4742399-4742617 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T183726 NZ_CP087168:4870372-4870650 [Pseudomonas sp. B21-048]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTACGAAACAGGTTCGACTCGTGGGATTCGGGCAGATCATGC
GAAACGTCTGGCTCGCATGCTGCAGTTCATGGATCGCGCAGTGGCCTCTGACGACCTCGACCTTCCGGGATGGCGATTAC
ACCCGCTCAAGGGTGATCTGATTGAATATTGGTCACTCAGCGTTTCGGGGAACTGGCGGGTTATTTTTCGTTTTATTGGT
TCGGATATCGAATTGGTCGACTATCTGGACTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT183726 WP_000344800.1 NZ_CP065970:4741999-4742373 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT183726 NZ_CP087168:4870665-4870952 [Pseudomonas sp. B21-048]
ATGCCCATGCACAACCCGCCACACCCTGGCGAAACACTCTTGATGGATGTATTGCCCGAGCTTGGTATCAGTGTCACCGA
ACTGGCCAGGCACCTTGGATTCGCCCGCCCGCATCTTTCGCGAGTGCTGCACGGCCATGCGCCCATCAGTCCGGACCTGG
CGGTACGTCTGGAGCGGGCTGGAATCGGTAAGGCCCGGATGTGGTTAGGAGTTCAGACTGACTACGATCTCTGGCAAGCG
GAGCAGCGTGAGCAACCCTTCATCGAACCGATTGCTATCCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References