Detailed information of TA system TA183715

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2866660..2867491
• Replicon: chromosome
• Accession: NZ_CP065970
• Organism: Escherichia coli strain FDAARGOS_1058

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: I6I15_RS14180
• Protein ID: WP_000854814.1
• Coordinates: 2866660..2867034 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: I6I15_RS14185
• Protein ID: WP_001285585.1
• Coordinates: 2867123..2867491 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I15_RS14140	2862056..2863222	+	1167	WP_000830163.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
I6I15_RS14145	2863341..2863814	+	474	WP_001105393.1	DNA gyrase inhibitor SbmC	-
I6I15_RS14150	2864012..2865070	+	1059	WP_001200905.1	FUSC family protein	-
I6I15_RS14155	2865242..2865571	+	330	WP_000450409.1	DUF496 family protein	-
I6I15_RS14160	2865672..2865854	-	183	WP_001016348.1	hypothetical protein	-
I6I15_RS14165	2865926..2866054	+	129	Protein_2641	transposase domain-containing protein	-
I6I15_RS14170	2866343..2866456	-	114	WP_001161660.1	DUF957 domain-containing protein	-
I6I15_RS14175	2866469..2866663	-	195	WP_000988600.1	hypothetical protein	-
I6I15_RS14180	2866660..2867034	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
I6I15_RS14185	2867123..2867491	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I6I15_RS14190	2867565..2867786	-	222	WP_000692323.1	DUF987 domain-containing protein	-
I6I15_RS14195	2867849..2868325	-	477	WP_001186726.1	RadC family protein	-
I6I15_RS14200	2868341..2868820	-	480	WP_000860087.1	antirestriction protein	-
I6I15_RS14205	2868902..2869720	-	819	WP_001234629.1	DUF945 domain-containing protein	-
I6I15_RS14210	2869820..2870053	-	234	WP_001119729.1	DUF905 family protein	-
I6I15_RS14215	2870132..2870587	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T183715 WP_000854814.1 NZ_CP065970:c2867034-2866660 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T183715 NZ_CP087167:c324205-323807 [Pseudomonas atacamensis]
ATGATCAAGTACATGCTGGATACAAATATCTGCATCTTCACCATCAAGAACAAACCACAAATTGTCCGGGAAGCCTTCAA
TCGACATTCCGGGCAGTTGTGCATCAGCGCTATCACGCTTATGGAGCTTATTTACGGTGCTGAAAAGTCGGCGGCCCCGG
AGAAAAATCTGGCAATTGTTGAAGGATTTGTCGCGCGACTGGATGTCTTGCCTTTCGATAACGACGCAGCCGCACAGGCC
GGGATGGTTCGATCTGAACTCGCGAAGGCTGGAACGCCGATCGGGCCATACGACCAAATGATCGCTGGCCACGCACGCTC
ACTTGGACTCATTGTAGTGACGAACAACGTACGGGAGTTTCAACGCGTTTCCGGTTTGCGAATCGAGGACTGGGTTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT183715 WP_001285585.1 NZ_CP065970:c2867491-2867123 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT183715 NZ_CP087167:c324435-324205 [Pseudomonas atacamensis]
ATGGAGCAGACCACAATTTTCATGAGCAACAGAAGTCAGGCAGTCCGACTGCCAAAGGCAGTTGCGATGCCCGGCGACGT
TAAACGAGTGGAAGTCATCGCGATTGGCAGAACACGCGTCATTACGCCTGCTGGAGAAAGCTGGGATAGCTGGTTTGATG
GCGACAGCGTCAGCCCTGATTTCATGGCTAACCGTGAGCAACCCACTGAACAGGAGCGCGAGTCGTTCTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60