Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1927675..1928329 Replicon chromosome
Accession NZ_CP065970
Organism Escherichia coli strain FDAARGOS_1058

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I6I15_RS09810 Protein ID WP_000244777.1
Coordinates 1927922..1928329 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6I15_RS09805 Protein ID WP_000354046.1
Coordinates 1927675..1927941 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I15_RS09780 1922963..1923706 + 744 WP_000951948.1 SDR family oxidoreductase -
I6I15_RS09785 1923763..1925196 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
I6I15_RS09790 1925241..1925552 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
I6I15_RS09795 1925716..1926375 + 660 WP_000250274.1 hemolysin III family protein -
I6I15_RS09800 1926452..1927432 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I6I15_RS09805 1927675..1927941 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6I15_RS09810 1927922..1928329 + 408 WP_000244777.1 toxin CptA Toxin
I6I15_RS09815 1928369..1928890 - 522 WP_001055874.1 flavodoxin FldB -
I6I15_RS09820 1929002..1929898 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6I15_RS09825 1929923..1930633 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6I15_RS09830 1930639..1932372 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T183710 WP_000244777.1 NZ_CP065970:1927922-1928329 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T183710 NZ_CP087166:1736590-1736802 [Pseudomonas sp. B21-051]
GTGCAAAGCAGGCTATTGATCAAGGAGCTGGAGGAGGCAGGCTGGACGCTGGATCGAGTCACCGGCAGTCATCACATCTT
CACGCATCGTTACAACCCGTACACCATTCCCGTTCCCCATCCGAAAAAGGATTTACCGCTGGGGACGGTCAAAAGCATCA
GGAGGCGTGCCGGGTTGTACCACCCGCCAGCCAGCAACAAGGGAGATCCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT183710 WP_000354046.1 NZ_CP065970:1927675-1927941 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT183710 NZ_CP087166:1736802-1737206 [Pseudomonas sp. B21-051]
ATGCAATATCCGATCTGCATCGAATGGGGTGACGACAACACCGCCATCGGCATTCAGATACCCGACATTCCCGGCGCCGT
CACGGCTGGGGACACCTTCGAGGACGCCTATAACGCCGCTGTTGAAGTCGCCCATCTCATGCTGCAGGAGATGGCGGCGG
ACGGGGAATCTATTCCCATGCCGACATCGGCAGCGGCGCATCGCAATAATCCGGAGTTTGCCGACATGGGCTGGGGGATG
CTTGAGCTGGATATCTCCCCGTACATGGGCAAGACCGAGAAGGTCAACGTGACGTTGCCTGGCTATGTGATCCAGCGCAT
TGATCGCTATGTGCGTGAACACAATGTCAAAAGCCGCTCTTCCTTTCTGGCAGATGCGGCGATGGAAAAACTGGTTCGGT
ATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References