Detailed information of TA system TA183709

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1753144..1753942
• Replicon: chromosome
• Accession: NZ_CP065970
• Organism: Escherichia coli strain FDAARGOS_1058

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: I6I15_RS09025
• Protein ID: WP_000854899.1
• Coordinates: 1753144..1753521 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6I15_RS09030
• Protein ID: WP_001315619.1
• Coordinates: 1753568..1753942 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I15_RS08990	1748208..1748807	+	600	WP_001255040.1	type II secretion system minor pseudopilin GspJ	-
I6I15_RS08995	1748810..1749787	+	978	WP_000633220.1	type II secretion system minor pseudopilin GspK	-
I6I15_RS09000	1749784..1750962	+	1179	WP_000094970.1	type II secretion system protein GspL	-
I6I15_RS09005	1750964..1751500	+	537	WP_000942786.1	GspM family type II secretion system protein YghD	-
I6I15_RS09010	1751781..1752353	-	573	WP_001290246.1	DUF4942 domain-containing protein	-
I6I15_RS09015	1752450..1752647	-	198	WP_000839281.1	DUF957 domain-containing protein	-
I6I15_RS09020	1752659..1753147	-	489	WP_000779174.1	hypothetical protein	-
I6I15_RS09025	1753144..1753521	-	378	WP_000854899.1	TA system toxin CbtA family protein	Toxin
I6I15_RS09030	1753568..1753942	-	375	WP_001315619.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6I15_RS09035	1754105..1754326	-	222	WP_000692311.1	DUF987 domain-containing protein	-
I6I15_RS09040	1754389..1754865	-	477	WP_001186770.1	RadC family protein	-
I6I15_RS09045	1754881..1755366	-	486	WP_000849576.1	antirestriction protein	-
I6I15_RS09050	1755421..1756239	-	819	WP_115200311.1	DUF945 domain-containing protein	-
I6I15_RS09055	1756339..1756572	-	234	WP_001119729.1	DUF905 family protein	-
I6I15_RS09060	1756651..1757106	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14227.22 Da
• Isoelectric Point: 6.8438

>T183709 WP_000854899.1 NZ_CP065970:c1753521-1753144 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADECVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T183709 NZ_CP087166:c1484611-1484432 [Pseudomonas sp. B21-051]
GTGAATAGCCGCTTTTTAATCGGTCAACTCGTCGCAGACGGCTGGTATCTGGTGCGGGTCAAAGGCAGTCATCATCACTT
TAAACATCCCATCAAGCCTGGACTGGTGACGGTGCCGCACCCGAAGAAAGACCTGCTAAAGAAAACGGCCATCAGCATTT
TGAAACAGGCGCTGCTCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13749.53 Da
• Isoelectric Point: 6.6249

>AT183709 WP_001315619.1 NZ_CP065970:c1753942-1753568 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQTPATTS

Nucleotide

• Length: 375 bp

>AT183709 NZ_CP087166:c1484395-1483988 [Pseudomonas sp. B21-051]
ATGCTTTACCCGATCGCAATTTCAATAGGTGATGAAGAACACGCTTGGGGAGTGGAAGTGCCGGATATTCCCGGCTGTTT
TTCTGCCGGCGATGATCTGGATGATGCCGTCGCAATGGCTCGCGAAGCCATTGAGGGTCATTTCGAAATTCTGGCAGAGG
ACGGTTCGCCGATCCCGCCAGCCAGCAAGCTTGGCGTGCACGTAGTGAACCCGCAGTATGCCGGTTGTGCCTGGGCTGTG
GTTGATATCGACGTTACCAAATACCTGGGCAAGGCGCAGAAACTCAACATCACGCTGCCCGGCCATCTGCTCAACCGCAT
TGATGAATATGTGCTTCATCACCCGGAAGAGAAAAGTCGTTCGGGGTTTCTGGCTTCGGCAGCGTTGAAGGTCTTGCAGC
AAGGTTGA