Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1240782..1241582 Replicon chromosome
Accession NZ_CP065970
Organism Escherichia coli strain FDAARGOS_1058

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag I6I15_RS06490 Protein ID WP_000342449.1
Coordinates 1241055..1241582 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag I6I15_RS06485 Protein ID WP_001277108.1
Coordinates 1240782..1241048 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I15_RS06465 1236439..1237107 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
I6I15_RS06470 1237100..1238158 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
I6I15_RS06475 1238403..1239257 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
I6I15_RS06480 1239529..1240632 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
I6I15_RS06485 1240782..1241048 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
I6I15_RS06490 1241055..1241582 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
I6I15_RS06495 1241579..1241962 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
I6I15_RS06500 1242386..1243495 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
I6I15_RS06505 1243543..1244469 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
I6I15_RS06510 1244466..1245743 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
I6I15_RS06515 1245740..1246507 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T183706 WP_000342449.1 NZ_CP065970:1241055-1241582 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T183706 NZ_CP087166:c304756-304442 [Pseudomonas sp. B21-051]
ATGATTTTTATTGAAACTCGAATCTTTACCCGTCGAGTAAAAGAGCTGCTGGATGATGACACGTACGCCGCTTTTCAGAA
GCAACTGGTTGTAAGCCCTTCGATCGGCGATGTAATTGAAGGCACAGGCGGGATTCGCAAAACCCGAATCGCAGCGAGAG
GACACGGCAAGCAAGGCGGAGCACGGGTTATTTACTACCACTTCGTTTCGGCGTCACAAATCGGGTTATTGATGATCTAC
CCTAAAAATGAACAACACGATCTGTCCAGCGATGAGCGTAAAGCACTCAAGGTCTTGATCGAAAAATGGAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT183706 WP_001277108.1 NZ_CP065970:1240782-1241048 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT183706 NZ_CP087166:c304439-304152 [Pseudomonas sp. B21-051]
ATGAGCAAATTTTTTGATGAGCTGATGGAAAGTGTGCAGGAGATGGATGAGATCGTGCGAGGCGAGCGCACACCTTCTCG
CGAATTCCACGTTGATGCATTGCAGGTCAAAGAAATTCGTAAAGCTACCGGTTTGACCCAGGCCAAATTTGCGGCACTGA
TCGACGTCCAACTCGGCACTCTACGAAATTGGGAACAGGGTCGTCGCGAGCCAACCGGGCCGGCAAAAGCTTTGCTCAAG
GCCATTCATAACGACCCGGTTCATGTTTTGATTGCATTGGCTCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References