Detailed information of TA system TA183697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2286641..2287227
• Replicon: chromosome
• Accession: NZ_CP065966
• Organism: Pseudomonas aeruginosa strain FDAARGOS_1041

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: I6H98_RS10795
• Protein ID: WP_003120987.1
• Coordinates: 2286928..2287227 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: I6H98_RS10790
• Protein ID: WP_003448662.1
• Coordinates: 2286641..2286931 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H98_RS10770	2281792..2282001	+	210	WP_003105733.1	cold-shock protein	-
I6H98_RS10775	2282223..2284112	+	1890	WP_016851610.1	hypothetical protein	-
I6H98_RS10780	2284109..2286085	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
I6H98_RS10785	2286226..2286570	+	345	WP_016851612.1	hypothetical protein	-
I6H98_RS10790	2286641..2286931	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
I6H98_RS10795	2286928..2287227	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H98_RS10800	2287429..2288553	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
I6H98_RS10805	2288553..2290262	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
I6H98_RS10810	2290266..2291591	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
I6H98_RS10815	2291581..2292114	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2259662..2362498	102836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T183697 WP_003120987.1 NZ_CP065966:c2287227-2286928 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T183697 NZ_CP087162:c8522-8223 [Pseudomonas sp. CIP-10]
ATGAAAGAACAATTTTACGACTACGATCCCGCTGATGCGCTGACCGATGCTGAGTCCATCGCAGTATTCATGGAGGACGC
ATTCGAAACAGGCGATGCGGCCTATATCGCAAAATGCCTGGGTGTCGTGGCCCGCGCCAAAGGGATGACCGCCCTTGCCA
ATGAATCTGGCCTTTCCCGCGAACACCTTTACCGGTCGTTCTCGGACAAGGGCAATCCGACCTTGAAAACCCTGCTGGCC
GTGATGCGTGCCGTTGGCCTGGACCTGACAACACGCAAGCACGTTGAACCGGCACACTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT183697 WP_003448662.1 NZ_CP065966:c2286931-2286641 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT183697 NZ_CP087162:c8821-8519 [Pseudomonas sp. CIP-10]
ATGAAGACAATTAAACAAACCGCCACGTACAGGAACTGGGAACGCAAGCTGCGAGACAAGCAGGCCAAAGCCATCATCGC
TGCGCGAGTCTTCCGCGTTGCGCATGGTCTGCTTGGAGACGTTCAACCAGTGGGCCAAGGGATCAGCGAACTGCGTATCC
ACCACGGCCCCGGCTACCGCGTGTATTTCCAGCAGCGCGGTAACCAGCTGGTCCTGTTGCTGTGTGGTGGGGACAAGAGC
AGCCAAGCGCGGGATATCGAAACCGCCAAGTCCTTGGCCAGCCAGTGGAGTGACGATGAATGA

Structures

Toxin

• AlphaFold DB: G8CP73