Detailed information of TA system TA183696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 1889649..1890257
• Replicon: chromosome
• Accession: NZ_CP065966
• Organism: Pseudomonas aeruginosa strain FDAARGOS_1041

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A1B1XUZ2
• Locus tag: I6H98_RS08940
• Protein ID: WP_003123043.1
• Coordinates: 1889649..1889996 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: I6H98_RS08945
• Protein ID: WP_003114155.1
• Coordinates: 1890006..1890257 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H98_RS08915	1885925..1887157	+	1233	WP_016852057.1	phosphoadenosine phosphosulfate reductase family protein	-
I6H98_RS08920	1887168..1887344	+	177	WP_016852058.1	hypothetical protein	-
I6H98_RS08925	1887341..1887709	+	369	WP_016852059.1	ASCH domain-containing protein	-
I6H98_RS08930	1887902..1888105	+	204	WP_003098423.1	AlpA family phage regulatory protein	-
I6H98_RS08935	1888112..1889314	-	1203	WP_016852061.1	integrase family protein	-
I6H98_RS08940	1889649..1889996	-	348	WP_003123043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H98_RS08945	1890006..1890257	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6H98_RS08950	1890471..1891454	-	984	WP_016852062.1	tyrosine-type recombinase/integrase	-
I6H98_RS08955	1891454..1892746	-	1293	WP_003115206.1	hypothetical protein	-
I6H98_RS08960	1893005..1894267	-	1263	WP_023093179.1	hypothetical protein	-
I6H98_RS08965	1894269..1894619	-	351	WP_003159569.1	DUF2523 domain-containing protein	-
I6H98_RS08970	1894629..1895234	-	606	WP_014603242.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1766117..1901580	135463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12990.74 Da
• Isoelectric Point: 4.4212

>T183696 WP_003123043.1 NZ_CP065966:c1889996-1889649 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T183696 NZ_CP087161:339432-339809 [Pseudomonas sp. CIP-10]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT183696 WP_003114155.1 NZ_CP065966:c1890257-1890006 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT183696 NZ_CP087161:339790-340125 [Pseudomonas sp. CIP-10]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1B1XUZ2

Antitoxin

• AlphaFold DB: A0A0B0C355