Detailed information of TA system TA183687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 234693..235215
• Replicon: plasmid pKP2509-MCR
• Accession: NZ_CP065953
• Organism: Klebsiella pneumoniae strain KP2509

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: JDE60_RS29375
• Protein ID: WP_004181778.1
• Coordinates: 234931..235215 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A5Q9LM53
• Locus tag: JDE60_RS29370
• Protein ID: WP_025368601.1
• Coordinates: 234693..234941 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDE60_RS29350 (JDE60_29325)	229893..230714	-	822	WP_046664185.1	hypothetical protein	-
JDE60_RS29355 (JDE60_29330)	230776..231129	-	354	WP_004181774.1	hypothetical protein	-
JDE60_RS29360 (JDE60_29335)	231274..232269	-	996	WP_156658702.1	hypothetical protein	-
JDE60_RS29365 (JDE60_29340)	232603..234402	-	1800	WP_042935572.1	ATP-dependent helicase	-
JDE60_RS29370 (JDE60_29345)	234693..234941	+	249	WP_025368601.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JDE60_RS29375 (JDE60_29350)	234931..235215	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JDE60_RS29380 (JDE60_29355)	235445..236671	+	1227	WP_042935575.1	RNA-guided endonuclease TnpB family protein	-
JDE60_RS29385 (JDE60_29360)	236957..237382	-	426	WP_040120489.1	IS200/IS605 family transposase	-
JDE60_RS29390 (JDE60_29365)	237419..238645	+	1227	WP_025368604.1	RNA-guided endonuclease TnpB family protein	-
JDE60_RS29395 (JDE60_29370)	238711..239837	-	1127	Protein_255	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IIa / blaTEM-1B / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / mcr-1.1	-	1..317010	317010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T183687 WP_004181778.1 NZ_CP065953:234931-235215 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T183687 NZ_CP087152:c124607-124164 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8906.12 Da
• Isoelectric Point: 3.8206

>AT183687 WP_025368601.1 NZ_CP065953:234693-234941 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT183687 NZ_CP087152:c124834-124604 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A5Q9LM53