Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 43720..44246 Replicon plasmid pKP2509-MCR
Accession NZ_CP065953
Organism Klebsiella pneumoniae strain KP2509

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JDE60_RS28415 Protein ID WP_000323025.1
Coordinates 43959..44246 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JDE60_RS28410 Protein ID WP_000534858.1
Coordinates 43720..43959 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JDE60_RS28390 (JDE60_28370) 38729..38989 - 261 WP_070083041.1 hypothetical protein -
JDE60_RS28395 (JDE60_28375) 39779..41119 + 1341 WP_000589001.1 ISNCY family transposase -
JDE60_RS28400 (JDE60_28380) 41762..42334 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
JDE60_RS28405 (JDE60_28385) 42534..43457 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
JDE60_RS30440 43591..43695 - 105 Protein_54 hypothetical protein -
JDE60_RS28410 (JDE60_28390) 43720..43959 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JDE60_RS28415 (JDE60_28395) 43959..44246 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JDE60_RS28420 (JDE60_28400) 44318..44476 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
JDE60_RS28425 (JDE60_28405) 45082..46056 + 975 WP_069127087.1 Hok/Gef family protein -
JDE60_RS28430 (JDE60_28410) 46123..46458 + 336 WP_095465480.1 hypothetical protein -
JDE60_RS28435 (JDE60_28415) 46720..47112 + 393 WP_211709437.1 hypothetical protein -
JDE60_RS28440 (JDE60_28420) 47557..47934 - 378 WP_029884711.1 hypothetical protein -
JDE60_RS28445 (JDE60_28425) 48261..48557 + 297 WP_094313394.1 hydrogenase expression/formation protein HypD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IIa / blaTEM-1B / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / mcr-1.1 - 1..317010 317010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T183686 WP_000323025.1 NZ_CP065953:43959-44246 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T183686 NZ_CP087152:c84563-84093 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT183686 WP_000534858.1 NZ_CP065953:43720-43959 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT183686 NZ_CP087152:c85003-84560 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References